Structure of dermatan sulfate. 3. The hybrid structure of dermatan sulfate from umbilical cord.
Structure of dermatan sulfate. 3. The hybrid structure of dermatan sulfate from umbilical cord.
复制标题
硫酸皮肤素的结构。
DOI：
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发表时间：
1968
影响因子：
4.8
通讯作者：
L. Fransson
中科院分区：
文献类型：
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作者：
L. Fransson
本文对从脐带中提取的硫酸皮肤素与猪皮硫酸皮肤素的性质进行了比较。像后者的多糖，脐带硫酸皮肤素被发现是一个混合物含有L-艾杜糖醛酸和D-葡萄糖醛酸;而半乳糖胺单位的猪皮硫酸皮肤素是专门的4-硫酸化，脐带多糖含有相当大比例的6-硫酸化半乳糖胺残基。后者的组件优先位于邻近的D-葡萄糖醛酸部分，而4-硫酸半乳糖胺单位普遍存在于L-艾杜糖醛酸的部分的分子。然而，获得的证据表明，与l-艾杜糖醛酸部分相邻的一些半乳糖胺残基是6-硫酸化的。该结论是基于睾丸透明质酸酶降解后获得的杂合八糖的表征。这些八糖均具有如下所示的单糖序列，含有位于还原和非还原末端的2个软骨素6-硫酸酯二糖单元，和2个主要为4-硫酸酯的含l-艾杜糖醛酸的内部二糖单元。然而，后者二糖中约三分之一似乎在6位硫酸化。[see序列PDF]
Abstract The properties of dermatan sulfate, isolated from umbilical cord, have been compared with those of pig skin dermatan sulfate. Like the latter polysaccharide, umbilical cord dermatan sulfate was found to be a hybrid containing both l-iduronic and d-glucuronic acid; whereas the galactosamine units of pig skin dermatan sulfate were exclusively 4-sulfated, the umbilical cord polysaccharide contained a considerable proportion of 6-sulfated galactosamine residues. The latter components were preferentially located adjacent to d-glucuronic acid moieties, while 4-sulfated galactosamine units were prevalent in l-iduronic acid-containing sections of the molecule. Evidence was obtained, however, indicating that some of the galactosamine residues adjacent to l-iduronic acid moieties were 6-sulfated. This conclusion was based on the characterization of hybrid octasaccharides obtained after degradation with testicular hyaluronidase. These octasaccharides which all had the monosaccharide sequence shown below contained 2 chondroitin 6-sulfate disaccharide units located in the reducing and nonreducing termini, and 2 l-iduronic acid-containing internal disaccharide units which were largely 4-sulfated. Approximately one-third of the latter disaccharides, however, appeared to be sulfated in position 6. [see PDF for sequence]