EXOPOLYSACCHARIDE COLANIC ACID AND ITS OCCURRENCE IN ENTEROBACTERIACEAE
EXOPOLYSACCHARIDE COLANIC ACID AND ITS OCCURRENCE IN ENTEROBACTERIACEAE
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DOI：
10.1128/jb.100.3.1187-1193.1969
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发表时间：
1969-01-01
影响因子：
3.2
通讯作者：
WILKINSON, JF
中科院分区：
文献类型：
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作者：
GRANT, WD;SUTHERLAND, IW;WILKINSON, JF
对沙门氏菌属、埃希氏菌属和需氧菌属菌株的研究表明，在适当的条件下，许多菌株产生一种成分相同的胞外多糖粘液，根据其化学成分和对某些噬菌体诱导的解聚酶的敏感性，已将其鉴定为大肠杆菌酸。化学分析表明，该多糖除了含有葡萄糖、半乳糖、岩藻糖和葡萄糖醛酸外，还含有O-乙酰基。除含葡糖醛酸的寡糖外，温和的酸水解还导致β-葡糖基岩藻糖的分离。许多菌株被发现在正常生长条件下或在有利于多糖合成的条件下合成柯拉尼酸，而其他菌株仅在控制机制为去阻遏的bypb-氟苯丙氨酸时合成柯拉尼酸。
A study of strains from the generaSalmonella, Escherichia, andAerobacterhas shown that under appropriate conditions many strains produce an exopolysaccharide slime of identical composition, which has been identified as colanic acid on the basis of its chemical composition and its sensitivity to certain bacteriophage-induced depolymerase enzymes. Chemical analysis shows that the polysaccharide containsO-acetyl groups in addition to the sugars glucose, galactose, fucose, and glucuronic acid. Mild acid hydrolysis has led to the isolation of a β-glucosylfucose in addition to glucuronic acid containing oligosaccharides. Many strains were found to synthesize colanic acid under normal conditions of growth or under conditions favoring polysaccharide synthesis, whereas others only synthesized colanic acid when the control mechanism was derepressed byp-fluorophenylalanine.