Genetic dissociation of acquisition and memory strength in the heat-box spatial learning paradigm in Drosophila
Genetic dissociation of acquisition and memory strength in the heat-box spatial learning paradigm in Drosophila
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DOI：
10.1101/lm.45506
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发表时间：
2006-01-01
影响因子：
2
通讯作者：
Zars, T
中科院分区：
文献类型：
--
作者：
Diegelmann, S;Zars, M;Zars, T
记忆呼叫有不同的强度，很大程度上取决于遇到的干扰强度。强化物和记忆强度之间的关系在渐进记忆曲线中很明显，渐进线的水平与强化物的强度相关。虽然这可能是记忆形成的一个基本特性，但人们对记忆强度是如何确定的知之甚少。果蝇不同水平的记忆成绩可以在全操作性热箱条件反射范式中测量。在这种空间学习模式中，苍蝇学习并记住避免与首选范围之外的温度相关的暗室的一半。强化温度对野生型果蝇的学习水平有很强的影响，较高的温度会诱导更强的记忆。此外，两个突变改变记忆采集曲线，改变采集速率或渐近记忆水平。芜菁突变，影响一个类型的腺苷酸环化酶，降低收购率。相反，修改所有ABC转运蛋白的白色突变限制了渐近记忆。白色突变不会对经典嗅觉条件反射产生负面影响，但实际上会在低强化水平下提高表现。因此，记忆获得/记忆强度和经典的嗅觉/操作性空间记忆在遗传上是分离的。一个概念模型的操作性条件反射和水平，芜菁甘蓝和白色影响条件反射的建议。
Memories call have different strengths, largely dependent on the intensity of reinforcers encountered. The relationship between reinforcement and memory strength is evident in asymptotic memory curves, with the level of the asymptote related to the intensity of the reinforcer. Although this is likely a fundamental property of memory formation, relatively little is known of how memory strength is determined. Memory performance at different levels in Drosophila call be measured in all operant heat-box conditioning paradigm. In this spatial learning paradigm, flies learn and remember to avoid one-half of a dark chamber associated with a temperature outside of the preferred range. The reinforcement temperature has a strong effect on the level of learning in wild-type flies, with higher temperatures inducing stronger memories. Additionally, two mutations alter memory-acquisition curves, either changing acquisition rate or asymptotic memory level. The rutabaga mutation, affecting a typed adenylyl cyclase, decreases the acquisition rate. In contrast, the white mutation, modifying all ABC transporter, limits asymptotic memory. The white mutation does not negatively affect classical olfactory conditioning but actually improves performance at low reinforcement levels. Thus, memory acquisition/memory strength and classical olfactory/operant spatial memories call be genetically dissociated. A conceptual model of operant conditioning and the levels at which rutabaga and white influence conditioning is proposed.