Activation of protein kinase C inhibits the expression of connective tissue growth factor.
Activation of protein kinase C inhibits the expression of connective tissue growth factor.
复制标题
蛋白激酶C的激活抑制结缔组织生长因子的表达。
DOI：
10.1006/bbrc.2000.3314
复制
发表时间：
2000
影响因子：
3.1
通讯作者：
Karnovsky,MJ
中科院分区：
文献类型：
--
作者：
Fan,WH;Karnovsky,MJ
结缔组织生长因子（CTGF）是一个38个半胱氨酸残基保守的蛋白质家族成员。尽管近年来这些蛋白质被赋予了多种重要的生物学功能，但其大多数成员的基因表达调控实际上是未知的。我们研究了蛋白激酶C（PKC）和酪氨酸激酶对CTGF表达的影响，发现在mRNA水平上，激活PKC可抑制CTGF的表达，而抑制PKC和酪氨酸激酶则可促进CTGF的表达。我们进一步确定，新的和经典的PKC亚型的抑制是必要的，但不涉及非典型亚型。我们的数据表明，由PKC和酪氨酸激酶对丝氨酸/苏氨酸和酪氨酸的磷酸化都抑制CTGF的表达。
Connective tissue growth factor (CTGF) is a member of a protein family in which 38 cysteine residues are conserved. Although a wide variety of important biological functions have been ascribed to these proteins in recent years, the regulation of their gene expression for most members is virtually unknown. We studied the effects of protein kinase C (PKC) and tyrosine kinase on the expression of CTGF and observed that at the mRNA level CTGF expression is inhibited by the activation of PKC, but stimulated by the inhibition of PKC and tyrosine kinase. We further determined that the novel and the classical PKC isoforms are needed for the inhibition, but the atypical isoforms are not involved. Our data suggest that phosphorylation on serine/threonine and tyrosine by PKC and by tyrosine kinase are all inhibitory to the expression of CTGF.