Demonstration by immunoperoxidase staining of hyperplasia of parafollicular cells in the thyroid gland in hyperparathyroidism.
Demonstration by immunoperoxidase staining of hyperplasia of parafollicular cells in the thyroid gland in hyperparathyroidism.
复制标题
通过免疫过氧化物酶染色证实甲状旁腺功能亢进症中甲状腺滤泡旁细胞的增生。
DOI：
10.1210/jcem-37-4-550
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发表时间：
1973
期刊：
影响因子：
--
通讯作者：
A. Tashjian
中科院分区：
文献类型：
--
作者：
V. Livolsi;C. Feind;P. Logerfo;A. Tashjian
用兔抗人降钙素对甲状旁腺腺瘤引起的高钙血症患者的甲状腺组织固定、脱蜡切片进行免疫过氧化物酶染色。在这些切片中，在滤泡旁位置鉴定出阳性染色细胞。这些细胞为卵圆形至矩形，偶尔显示单极胞质延伸。染色呈颗粒状，定位于细胞质。偶尔观察到这些细胞簇。正常甲状腺和各种甲状腺疾病患者的腺体的对照切片也被染色。在这些对照切片中，每50个低倍（×100）视野中识别出50个或更少的染色细胞，而在高钙血症患者甲状腺的可比切片中，计数到多达880个含降钙素的细胞。在14例甲状旁腺功能亢进患者中，36%的患者滤泡旁细胞增生。结论：甲状旁腺腺瘤是一种少见的恶性肿瘤。
ABSTRACT Immunoperoxidase staining, using rabbit antihuman calcitonin, was performed on fixed, deparaffinized sections of thyroid tissue from patients with hypercalcemia caused by parathyroid adenomas. In these sections, positively stained cells were identified in a parafollicular location. These cells were ovoid to rectangular, occasionally showing unipolar cytoplasmic extensions. The staining was granular in character and was localized to the cytoplasm. Occasional clusters of these cells were noted. Control sections of normal thyroid glands and of glands from patients with various thyroid disorders were also stained. In these control sections, 50 or fewer stained cells per 50 low power (×100) fields were identified, whereas in comparable sections of thyroid glands from hypercalcemic patients as many as 880 calcitonin-containing cells were counted. Hyperplasia of parafollicular cells was found in 36% of 14 hyperparathyroid patients studied. Neither parathyroid adenomas nor thymic remnants found in some o...