Sol-gel derived nano-structured zinc oxide film for sexually transmitted disease sensor
Sol-gel derived nano-structured zinc oxide film for sexually transmitted disease sensor
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DOI：
10.1039/b817562d
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发表时间：
2009-01-01
期刊：
影响因子：
4.2
通讯作者：
Malhotra, B. D.
中科院分区：
文献类型：
--
作者：
Ansari, Anees A.;Singh, Renu;Malhotra, B. D.
将一种针对淋病奈瑟菌的20聚硫代寡核苷酸探针（th-ssDNA）固定在溶胶-凝胶衍生的纳米结构氧化锌（ZnO）薄膜上，并将其浸渍在氧化铟锡（ITO）玻璃基板上，利用杂交技术制备了一种用于性传播疾病（淋病）检测的DNA生物传感器。利用x射线衍射、紫外可见、傅里叶变换红外、扫描电镜和电化学技术对该ths - ssdna - zno /ITO生物电极进行了表征，结果表明该电极的线性范围为0.000524 fmol ~ 0.524 nmol， 60 s内的检出限为0.000704 fmol。
A 20-mer thiolated oligonucleotide probe (th-ssDNA) specific to Neisseria gonorrhoeae immobilized onto a sol-gel derived nano-structured zinc oxide (ZnO) film dip-coated onto an indium-tin-oxide (ITO) glass substrate has been used for the fabrication of a DNA biosensor for sexually transmitted disease (gonorrhoea) detection using hybridization technique. The results of characterization studies carried out on this th-ssDNA-ZnO/ITO bioelectrode using X-ray diffraction, UV-Visible, Fourier-transform infrared, scanning electron microscopy and electrochemical techniques reveal the linearity as 0.000524 fmol-0.524 nmol, with a detection limit of 0.000704 fmol within 60 s.