Activation of host lymphocytes cultured with cancer cells treated with neuraminidase.
Activation of host lymphocytes cultured with cancer cells treated with neuraminidase.
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与经神经氨酸酶处理的癌细胞一起培养的宿主淋巴细胞的激活。
DOI：
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发表时间：
1971
期刊：
影响因子：
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通讯作者：
M. Waters
中科院分区：
文献类型：
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作者：
E. Watkins;Y. Ogata;L. L. Anderson;E. Watkins;M. Waters
对恶性肿瘤的免疫应答可以通过测量与恶性靶细胞一起培养的宿主淋巴细胞的母细胞转化来定量，其中合成多核苷酸的能力已经被阻断1 -3。我们已经使用这种类型的靶细胞培养物来研究用旨在增强宿主免疫应答的试剂预处理恶性靶细胞的效果。作为一项初步研究，我们已经评估了霍乱弧菌酶，神经氨酸酶，从癌细胞膜上去除唾液酸基团的作用。有证据表明，从实验性肿瘤的细胞表面去除这些糖基有利于与肿瘤细胞相关的抗原的免疫识别4 -6。我们观察到大鼠宿主外周血白细胞与神经氨酸酶预处理的Novikoif肝癌靶细胞培养后，淋巴母细胞反应增强。在研究的11个人中，有8个人对酶处理的恶性细胞表现出类似的反应。
IMMUNE response to malignancy can be quantified by measurement of blastic transformation of host lymphocytes cultured with malignant target cells in which the capacity to synthesize polynucleotides has been blocked1–3. We have used this type of target cell culture to study the effects of pretreatment of malignant target cells with agents intended to accentuate host immune response. As an initial study we have evaluated the action of the Vibrio cholerae enzyme, neuraminidase, which removes sialic acid groups from the cancer cell membrane. There is evidence that removal of such saccharide radicals from the cell surface of experimental tumours facilitates immune recognition of antigens associated with the tumour cells4–6. We have observed an accentuated lymphoblastic response of rat host peripheral blood leucocytes cultured with Novikoif hepatoma target cells pretreated with neuraminidase. Eight out of eleven humans studied have shown a similar response to enzyme treated malignant cells.