Molecular evolution of IgG subclass among nonhuman primates: Implication of differences in antigenic determinants among apes
Molecular evolution of IgG subclass among nonhuman primates: Implication of differences in antigenic determinants among apes
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DOI：
10.1007/bf02629608
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发表时间：
2002-10-01
期刊：
影响因子：
1.7
通讯作者：
Terao, K
中科院分区：
文献类型：
--
作者：
Asada, Y;Kawamoto, Y;Terao, K
通过竞争性ELISA测定了5种不同兔多克隆抗体对人IgG和IgG亚类（IgG1、IgG2、IgG3和IgG4）的交叉反应性，其中9种非人灵长类动物包括5种猿、3种旧世界猴和1种新世界猴。抗人IgG抗体与不同灵长类动物血浆的反应性与其与人之间的遗传距离密切相关。每种抗人IgG亚类抗体与来自旧世界和新世界猴的血浆均显示低交叉反应性。除长臂猿（Hylobates spp.）显示出与抗人IgG 2和IgG 3抗体的60%至100%的交叉反应性。另一方面，黑猩猩（Pan troglodytes和Pan paniscus）和猩猩（Pongo pygmaeus）血浆与抗人IgG I抗体显示100%交叉反应性，但大猩猩（Gorilla gorilla）和猩猩血浆未显示交叉反应性。黑猩猩和大猩猩血浆与抗人IgG4抗体的交叉反应性不同，黑猩猩为100%，大猩猩为50%，而猩猩和大猩猩血浆中未观察到交叉反应性。这些结果表明，在从长臂猿科分化为智人的同时，可能也出现了“人型”IgG亚类的分化，而且在大猿中IgG 1和IgG 4的分子进化与IgG 2和IgG 3的分子进化不同，这可能是由于在进化过程中猿的免疫功能发育不同所致。
The cross-reactivity of five different rabbit polyclonal antibodies to human IgG and IgG subclass (IgG1, IgG2, IgG3, and IgG4) was determined by competitive ELISA with nine nonhuman primate species including five apes, three Old World monkeys, and one New World monkey. As similar to those previously reported, the reactivity of anti-human IgG antibody with plasma from different primate species was closely related with phylogenic distance from human. Every anti-human IgG subclass antibody showed low cross-reactivity with plasma from Old World and New World monkeys. The plasma from all apes except for gibbons (Hylobates spp.) showed 60 to 100% of cross-reactivity with anti-human IgG2 and IgG3 antibodies. On the other hand, chimpanzee (Pan troglodytes and Pan paniscus) and orangutan (Pongo pygmaeus) plasma showed 100% cross-reactivity with anti-human IgG I antibody, but gorilla (Gorilla gorilla) and gibbon plasma showed no cross-reactivity. The chimpanzee and gorilla plasma cross-reacted with anti-human IgG4 antibody at different reactivity, 100% in chimpanzee and 50% in gorilla, but no cross-reactivity was observed in orangutan and gibbon plasma. These results suggest the possibilities that the divergence of "human-type" IgG subclasses might occur at the time of divergence of Homo sapience from Hylobatidae, and that the molecular evolution of IgG1 as well as IgG4 is different from that of IgG2 and IgG3 in great apes, this is probably caused by different in development of immune function in apes during the course of evolution.