Mechanical difference between white and gray matter in the rat cerebellum measured by scanning force microscopy
Mechanical difference between white and gray matter in the rat cerebellum measured by scanning force microscopy
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DOI：
10.1016/j.jbiomech.2010.07.002
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发表时间：
2010-11-16
影响因子：
2.4
通讯作者：
Guck, Jochen
中科院分区：
文献类型：
--
作者：
Christ, Andreas F.;Franze, Kristian;Guck, Jochen
组织的力学性质越来越被认为是细胞生理学和病理学的重要线索。然而，有一个稀疏的定量，高分辨率的数据对特定组织的机械性能。这对于中枢神经系统（CNS）尤其如此，其在制备和测量方面造成特别困难。我们已经准备了薄切片的脑组织适合压痕测量的微米尺度在一个接近原生状态。本文用扫描力显微镜测量了大鼠小脑的有效弹性模量，其空间分辨率为100 μ m。我们发现白色和灰质之间存在显著差异，在3 μ m压痕深度时，有效弹性模量分别为K=294 +/- 74和454 +/- 53 Pa（4只动物的n（g）= 245，n（w）=150，p < 0.05;误差为SD）。在较大的长度尺度上的许多其他的测量相比，我们的结果是恒定的压痕深度为2-4 μ m表示的制度的线性有效弹性模量。这些数据，评估与直接的机械测量，提供可靠的高分辨率的信息，并作为进一步的神经力学研究的发展，成人和受损的中枢神经系统组织的机械性能的定量基础。(C)2010爱思唯尔有限公司版权所有。
The mechanical properties of tissues are increasingly recognized as important cues for cell physiology and pathology. Nevertheless, there is a sparsity of quantitative, high-resolution data on mechanical properties of specific tissues. This is especially true for the central nervous system (CNS), which poses particular difficulties in terms of preparation and measurement. We have prepared thin slices of brain tissue suited for indentation measurements on the micrometer scale in a near-native state. Using a scanning force microscope with a spherical indenter of radius similar to 20 mu m we have mapped the effective elastic modulus of rat cerebellum with a spatial resolution of 100 mu m. We found significant differences between white and gray matter, having effective elastic moduli of K=294 +/- 74 and 454 +/- 53 Pa, respectively, at 3 mu m indentation depth (n(g) = 245, n(w)=150 in four animals, p < 0.05; errors are SD). In contrast to many other measurements on larger length scales, our results were constant for indentation depths of 2-4 mu m indicating a regime of linear effective elastic modulus. These data, assessed with a direct mechanical measurement, provide reliable high-resolution information and serve as a quantitative basis for further neuromechanical investigations on the mechanical properties of developing, adult and damaged CNS tissue. (C) 2010 Elsevier Ltd. All rights reserved.