Preliminary results comparing OIH, 99mTc-MAG3 and iothalamate in a rat model of renal ischemia.
Preliminary results comparing OIH, 99mTc-MAG3 and iothalamate in a rat model of renal ischemia.
复制标题
比较 OIH、99mTc-MAG3 和碘酞酸盐在肾缺血大鼠模型中的初步结果。
DOI：
10.1159/000418143
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发表时间：
1990
影响因子：
--
通讯作者：
TaylorJr,A
中科院分区：
文献类型：
--
作者：
Eshima,D;Greene,J;TaylorJr,A
方法本研究中使用的方法之前已详细描述过[6, 7]。简言之，对四组动物进行了研究，其中包括假缺血单肾对照组以及在释放夹子后立即以及24小时和72小时后进行研究的组。动物的手术准备如下：中线切口并分离左肾动脉；待核心温度恢复正常后，经股静脉静脉注射肝素50单位，1min后闭塞左肾动脉。将动物封闭并维持核心温度30分钟的缺血期。缺血期结束时进行右肾切除术。对照动物经历了相同的程序；然而，左肾动脉并未闭塞。立即对实验动物进行研究，并在松开夹子后 24 小时和 72 小时进行研究。
MethodsThe methods used in this study have been described previously in detail [6, 7]. Briefly, four groups of animals were studied, a sham ischemic single kidney control group and groups studied immediately and at 24 and 72 h after release of the clamp. The animals were prepared surgically as follows: a midline incision was made and the left renal artery was isolated; the core temperature was allowed to return to normal, then 50 units of heparin were given intravenously through the femoral vein and 1 min later the left renal artery was occluded. The animals were closed and the core temperature maintained for the 30-min ischemic period. At the end of the ischemic period a right nephrectomy was performed. The control animals underwent an identical procedure; however, the left renal artery was not occluded. The experimental animals were studied immediately and at 24 and 72 h after release of the clamp.