The effects of growth factors and phorbol esters on steroid biosynthesis in isolated human theca interna and granulosa-lutein cells in long term culture.
The effects of growth factors and phorbol esters on steroid biosynthesis in isolated human theca interna and granulosa-lutein cells in long term culture.
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DOI：
10.1210/jcem.79.1.8027214
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发表时间：
1994-07
期刊：
影响因子：
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通讯作者：
J. McAllister;W. Byrd;E. Simpson
中科院分区：
文献类型：
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作者：
J. McAllister;W. Byrd;E. Simpson
在本报告中，我们研究了生长因子和佛波酯对培养的人鞘细胞和颗粒叶黄素细胞中类固醇羟化酶活性的影响。用表皮生长因子 (EGF)、成纤维细胞生长因子 (FGF)、转化生长因子-β (TGF beta) 和十四烷酰佛波醇乙酸酯 (TPA) 处理鞘细胞，可抑制毛喉素和二丁酰 cAMP 刺激的 17 α 羟化酶活性以及 17 α 羟孕酮和脱氢表雄酮的产生。相比之下，FGF 和 TGF β 增强了 cAMP 刺激的 3β-羟基类固醇脱氢酶 (3β HSD) 活性，并且 EGF 治疗增强了 cAMP 刺激的孕酮产生。 cAMP 刺激的 3β HSD 活性不受 TPA (10 nmol/L) 处理的影响，但 TPA 抑制 cAMP 刺激的孕酮产生。 TPA 抑制 Basal 3 beta HSD 活性和孕酮产生。与EGF、FGF和TGFβ对17α-羟化酶表达的抑制作用相反，胰岛素和胰岛素样生长因子-I增强了毛喉素刺激的17α-羟化酶活性。在颗粒叶黄素细胞中，毛喉素刺激的芳香酶活性被 EGF、FGF 和 TPA 抑制。 TGF beta 对毛喉素刺激的芳香酶活性没有影响。 EGF、FGF 和 TGF β 不影响毛喉素刺激的孕酮分泌，而 TPA 治疗则抑制 cAMP 刺激的孕酮分泌。这些数据表明，生长因子可能差异性地调节发育中卵泡的人类鞘细胞和颗粒细胞中的 cAMP 依赖性过程。
In this report we examined the effects of growth factors and phorbol esters on steroid hydroxylase activity in cultured human thecal and granulosa-lutein cells. Treatment of thecal cells with epidermal growth factor (EGF), fibroblast growth factor (FGF), transforming growth factor-beta (TGF beta), and tetradecanoyl phorbol acetate (TPA) resulted in the inhibition of forskolin- and dibutyryl cAMP-stimulated 17 alpha-hydroxylase activity and 17 alpha-hydroxyprogesterone and dehydroepiandrosterone production. In contrast, cAMP-stimulated 3 beta-hydroxysteroid dehydrogenase (3 beta HSD) activity was enhanced by FGF and TGF beta, and treatment with EGF enhanced cAMP-stimulated progesterone production. cAMP stimulated 3 beta HSD activity was unaffected by TPA (10 nmol/L) treatment, yet TPA inhibited cAMP-stimulated progesterone production. Basal 3 beta HSD activity and progesterone production were inhibited by TPA. In contrast to the inhibitory actions of EGF, FGF, and TGF beta on 17 alpha-hydroxylase expression, insulin and insulin-like growth factor-I enhanced forskolin-stimulated 17 alpha-hydroxylase activity. In granulosa-lutein cells, forskolin-stimulated aromatase activity was suppressed by EGF, FGF, and TPA. TGF beta had no effect on forskolin-stimulated aromatase activity. EGF, FGF, and TGF beta did not affect forskolin-stimulated progesterone production, whereas treatment with TPA inhibited cAMP-stimulated progesterone secretion. These data suggest that growth factors may differentially regulate cAMP-dependent processes in human thecal and granulosa cells of the developing follicle.