Characterization of microtubule-associated proteins isolated from bovine adrenal gland.
Characterization of microtubule-associated proteins isolated from bovine adrenal gland.
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从牛肾上腺分离的微管相关蛋白的表征。
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
H. Sakai
中科院分区：
文献类型：
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作者：
S. Kotani;H. Murofushi;S. Maekawa;C. Sato;H. Sakai
我们研究了肾上腺髓质和皮质微管相关蛋白(MAP)的生化特征。通过紫杉醇依赖程序分离的肾上腺图谱的主要成分[Vallee，R.B.(1982)J.Cell.比奥尔。92,435-442]是几个位于高分子量区的多肽(High-MR MAP)和一个190000-MR多肽(190-kDa MAP)。在皮质MAP部分中，最显著的成分是190 kDa MAP，而延髓MAP部分则富含高MR MAP。同样来源的不含紫杉醇的两次循环微管蛋白也包含高MR图谱和190 kDa图谱。从电泳迁移率、热稳定性和免疫反应性来看，高MR图谱包含与哺乳动物脑MAP1和MAP2相同的蛋白质种类。190 kDa的MAP根据不同的标准被划分为不同于高MR MAP的MAP亚种。MAP组分具有将纯化的微管蛋白聚合成微管的能力，并且MAP的主要物种(高MR MAP和190 kDa MAP)与重组的微管共聚。Tau因子是哺乳动物大脑中的主要图谱之一，在肾上腺中似乎是一个次要的物种。
We investigated the biochemical characteristics of microtubule-associated proteins (MAPs) of both the adrenal medulla and the cortex. The major constituents of the adrenal MAPs isolated by the taxol-dependent procedure [Vallee, R. B. (1982) J. Cell. Biol. 92, 435-442] were several polypeptides in the high-molecular-mass region (high-Mr MAPs) and a 190000-Mr polypeptide (190-kDa MAP). In the cortex MAP fraction, the most prominent component was 190-kDa MAP, while the medulla MAP fraction was rich in high-Mr MAPs. Twice-cycled microtubule proteins prepared without taxol from the same sources also contained high-Mr MAPs and 190-kDa MAP. High-Mr MAPs contained protein species identical to MAP1 and MAP2 of mammalian brain as judged from electrophoretic mobility, heat-stability and immunoreactivity. 190-kDa MAP was classified as MAP subspecies distinct from high-Mr MAPs by several criteria. The MAP fractions had the ability to polymerize purified tubulin into microtubules, and the major MAP species (high-Mr MAPs and 190-kDa MAP) were found to cosediment with reconstituted microtubules. Tau factor, one of the major MAPs in the mammalian brain, appeared to be a minor species in the adrenal gland.
来自培养的 HeLa 细胞的微管相关蛋白。
DOI：
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发表时间：
1980
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Bulinski,JC;Borisy,GG
通讯作者：
Borisy,GG
DOI：
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发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Berk,BC;Hinkle,PM
通讯作者：
Hinkle,PM