Extracellular-regulated-kinase 5-mediated renal protection against ischemia-reperfusion injury.
Extracellular-regulated-kinase 5-mediated renal protection against ischemia-reperfusion injury.
复制标题
DOI：
10.1016/j.bbrc.2012.01.043
复制
发表时间：
2012-02-24
影响因子：
3.1
通讯作者：
Yang, Jay
中科院分区：
文献类型：
--
作者：
Kawakami, Tomoko;Park, Sang Won;Kaku, Ryuji;Yang, Jay
ERK5 是丝裂原激活蛋白激酶的成员，在肾脏中表达的表观分子质量 (~80 kDa) 比其他器官 (~120 kDa) 更小。封闭肽实验证实，蛋白质印迹上检测到的约 80 kDa 是抗 ERK5 抗体检测到的特异性条带。已知 ERK5 变体 ERK5a、b、c 和 T 的表达证实没有已知的剪接变体编码肾脏特异性~80 kDa 蛋白。然而，使用针对潜在剪接位点的引物进行的 RT-PCR 并未揭示肾脏中的新转录本。肾脏特异性 ERK5 免疫反应蛋白的较小分子量表明，这种细胞保护分子在肾脏中可能无法完全发挥作用。慢病毒介导的小鼠肾脏体内全长 ERK5 过表达提供了针对肾 IR 损伤的保护作用。肾脏特异性~80 kDa ERK5 的身份仍然未知，但更好地了解肾脏中 ERK5 的表达和翻译后加工可能会揭示一种新的肾脏保护策略。
ERK5, a member of the mitogen activated protein kinase, expressed in the kidneys was smaller (~80 kDa) in apparent molecular mass compared to other organs (~120 kDa). A blocking peptide experiment confirmed that the ~80 kDa detected on Western blots was a specific band detected by the anti-ERK5 antibody. Expression of the known ERK5 variants ERK5a, b, c, and T confirmed that none of the known splice variants encoded for the renal-specific ~80 kDa protein. However, RT-PCR with primers targeting the potential splice sites did not reveal a novel transcript in the kidney. The smaller molecular mass of the kidney-specific ERK5-immunoreactive protein suggested that this cyto-protective molecule may not be fully functional in the kidneys. Lentivirus-mediated in vivo overexpression of full length ERK5 in the mouse kidneys provided protection against renal IR injury. The identity of the renal-specific ~80 kDa ERK5 remains unknown but a better understanding of the ERK5 expression and post-translational processing in the kidneys may reveal a novel strategy for renal protection.
登录
查看更多内容
DOI：
10.1152/ajprenal.00124.2009
发表时间：
2010-01-01
影响因子：
4.2
作者：
Urushihara, Maki;Takamatsu, Masanori;Kagami, Shoji
通讯作者：
Kagami, Shoji
影响因子：
3.5
作者：
Cameron, SJ;Itoh, S;Abe, J
通讯作者：
Abe, J
影响因子：
19.6
作者：
Kim, Minjae;Chen, Sean W. C.;Park, Sang Won;Kim, Mihwa;D'Agati, Vivette D.;Yang, Jay;Lee, H. Thomas
通讯作者：
Lee, H. Thomas
影响因子：
14.8
作者：
Tiscornia, Gustavo;Singer, Oded;Verma, Inder M.
通讯作者：
Verma, Inder M.
影响因子：
4.8
作者：
Abe, J;Takahashi, M;Berk, BC
通讯作者：
Berk, BC