Phytochemical analyses of Ziziphus jujuba Mill. var. spinosa seed by ultrahigh performance liquid chromatography-tandem mass spectrometry and gas chromatography-mass spectrometry
Phytochemical analyses of Ziziphus jujuba Mill. var. spinosa seed by ultrahigh performance liquid chromatography-tandem mass spectrometry and gas chromatography-mass spectrometry
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大枣的植物化学分析。
DOI：
10.1039/c3an01478a
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发表时间：
2013-01-01
期刊：
影响因子：
4.2
通讯作者：
Jiang, Yueming
中科院分区：
文献类型：
--
作者：
Yang, Bao;Yang, Hongshun;Jiang, Yueming
齐齐夫斯枣厂。由于其对中枢神经系统紊乱的显著作用，红枣种子在医学领域受到了广泛的关注。次生代谢物的组成是影响该植物药用和商业品质的关键。采用超高效液相色谱-串联质谱法（UPLC-MS/MS）和气相色谱-质谱法（GC-MS）对枣子的植物化学特征进行了分析。UPLC-MS/MS信息鉴定出枣仁主要次生代谢产物为黄酮类c -苷、三萜酸和不饱和脂肪酸。UPLC-MS/MS鉴定的主要挥发性成分为白桦酸，GC-MS鉴定的主要挥发性成分为油酸。所有测试的样本都显示出相似的植物化学特征，但化学化合物的含量有所不同。主成分分析揭示了主要次生代谢物可以确定质量差异。结果表明，UPLC-MS/MS和GC-MS联用技术是检验枣子药用品质的有效方法。
Ziziphus jujuba Mill. var. spinosa (Z. jujuba) seeds have attracted much attention within the field of medicine due to their significant effects against disturbances of the central nervous system. Secondary metabolites composition is key to the influence of the pharmaceutical and commercial qualities of this plant. In this work, the phytochemical profile of Z. jujuba seeds was analysed by ultrahigh performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) and gas chromatography-mass spectrometry (GC-MS). The UPLC-MS/MS information identified the main secondary metabolites in Z. jujuba seeds, including flavonoid C-glycosides, triterpene acids and unsaturated fatty acids. The leading chemical identified by UPLC-MS/MS was betulinic acid, and oleic acid was the leading volatile from the GC-MS results. All the samples tested showed similar phytochemical profiles, but levels of the chemical compounds varied. Principal component analysis revealed the principal secondary metabolites that could define the differences in quality. It was confirmed that the combination of UPLC-MS/MS and GC-MS was an effective technique to demonstrate the pharmaceutical quality of Z. jujuba seeds.