Corynebacterium propinquum Keratitis Identified Using MALDI-TOF
Corynebacterium propinquum Keratitis Identified Using MALDI-TOF
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DOI：
10.1097/ico.0000000000000787
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发表时间：
2016-05-01
期刊：
影响因子：
2.8
通讯作者：
Mills, Richard A.
中科院分区：
文献类型：
--
作者：
Badenoch, Paul R.;O'Daniel, Lisa J.;Mills, Richard A.
目的：报告角膜感染，由于棒状杆菌propinquum确定使用基质辅助激光解吸/电离飞行时间（MALDI-TOF）methods.Methods：一个94岁的女子化脓性角膜炎。她的眼部病史包括Fuchs角膜营养不良、角膜移植和青光眼。革兰氏染色涂片显示棒状杆菌。用MALDI-TOF法检测培养液上的菌落数。通过RNA聚合酶亚基（rpoB）基因测序证实了propinquum。该患者接受了外用头孢唑林、庆大霉素和氟米龙治疗，最后一次就诊时，她的视力在没有辅助的情况下改善到20/160。结论：MALDI-TOF可以正确识别罕见且难以与相关物种区分的分离株。该方法的简便性和低运行成本将改善眼部感染的实验室诊断。
Purpose:To report a corneal infection due to Corynebacterium propinquum identified using the matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) method.Methods:A 94-year-old woman presented with suppurative keratitis. Her ocular history included Fuchs corneal dystrophy, corneal transplantation, and glaucoma. A gram-stained smear revealed coryneforms. Colonies on bacterial culture media were assayed by MALDI-TOF.Results:Identification of C. propinquum was confirmed by RNA polymerase subunit (rpoB) gene sequencing. The patient was treated with topical cefazolin, gentamicin, and fluorometholone, and her vision improved to 20/160 unaided when last seen.Conclusions:MALDI-TOF can correctly identify isolates that are both uncommon and difficult to distinguish from related species. The ease and low running costs of this method will improve the laboratory diagnosis of ocular infections.