Different regulation of hepatocyte behaviors between natural extracellular matrices and synthetic extracellular matrices by hepatocyte growth factor.
Different regulation of hepatocyte behaviors between natural extracellular matrices and synthetic extracellular matrices by hepatocyte growth factor.
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肝细胞生长因子对天然细胞外基质和合成细胞外基质之间肝细胞行为的不同调节。
DOI：
10.1002/jbm.a.31571
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
T. Akaike
中科院分区：
文献类型：
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作者：
T. Hoshiba;Mai Wakejima;C. Cho;G. Shiota;T. Akaike
生长因子和细胞外基质在肝细胞行为调控中的作用对肝组织工程的建立具有重要意义。特别是，生长因子和ECM之间的协作是肝组织工程的一大关注点。在这项研究中，肝细胞生长因子（HGF）的肝细胞反应进行了比较之间的天然ECM和合成的半乳糖携带聚合物：聚（N-对-乙烯基苄基-4-O-β-D-吡喃半乳糖-D-甘露酰胺）（PVLA）。肝细胞在HGF存在下在I型胶原蛋白和纤连蛋白涂覆的表面上增殖，而肝细胞在HGF存在下在层粘连蛋白-1-、PVLA-和聚-L-赖氨酸（PLL）涂覆的表面上形成球状体，而不活化增殖。HGF加速ECM沉积，特别是层粘连蛋白-10/11，在PVLA和PLL包被的表面上培养的肝细胞下方，并且沉积的层粘连蛋白-10/11激活整合素信号传导以与HGF信号传导协作。因此，应关注沉积的ECM分子，以明确HGF存在下肝细胞行为的机制。
The roles of growth factors and extracellular matrices (ECMs) in regulation of hepatocyte behaviors are very important for the establishment of liver-tissue engineering. Especially, collaboration between growth factors and ECMs is a big concern for liver-tissue engineering. In this study, the hepatocyte responses by hepatocyte growth factor (HGF) were compared between natural ECMs and a synthetic galactose-carrying polymer: poly(N-p-vinylbenzyl-4-O-beta-D-galactopyranosyl-D-gluconamide) (PVLA). Hepatocytes underwent proliferation on type I collagen- and fibronectin-coated surfaces in the presence of HGF, whereas hepatocytes formed spheroid on laminin-1-, PVLA-, and poly-L-lysine (PLL)-coated surfaces in the presence of HGF without the activation of proliferation. HGF accelerated ECM deposition, especially laminin-10/11, beneath the hepatocytes cultured on PVLA- and PLL-coated surfaces and the deposited laminin-10/11 activated integrin signaling to collaborate with HGF signaling. Therefore, the deposited ECM molecules should be focused to clear the mechanism of hepatocyte behaviors in the presence of HGF.