THE PROTEIN ENCODED BY A GROWTH ARREST-SPECIFIC GENE (GAS6) IS A NEW MEMBER OF THE VITAMIN-K-DEPENDENT PROTEINS RELATED TO PROTEIN-S, A NEGATIVE COREGULATOR IN THE BLOOD-COAGULATION CASCADE
THE PROTEIN ENCODED BY A GROWTH ARREST-SPECIFIC GENE (GAS6) IS A NEW MEMBER OF THE VITAMIN-K-DEPENDENT PROTEINS RELATED TO PROTEIN-S, A NEGATIVE COREGULATOR IN THE BLOOD-COAGULATION CASCADE
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DOI：
10.1128/mcb.13.8.4976
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发表时间：
1993-08-01
影响因子：
5.3
通讯作者：
SCHNEIDER, C
中科院分区：
文献类型：
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作者：
MANFIOLETTI, G;BRANCOLINI, C;SCHNEIDER, C
先前已经描述了一组生长停滞特异性基因（gas），其表达在血清诱导后被负调控（C.施奈德河，巴西-地M. King和L. Philipson，Cell 54：787 - 793，1988）。详细分析其中之一，气体6，报告在这里。gas6mRNA（2.6kb）在血清饥饿（在0.5%胎牛血清中48h）的NIH 3T3细胞中大量表达，但在胎牛血清或碱性成纤维细胞生长因子刺激后显著降低。gas6的人同源物也被克隆和测序，揭示了在IMR90人成纤维细胞中的高度同源性和相似的表达模式。计算机分析的蛋白质编码的小鼠和人类gas6 cDNA显示显着的同源性（43和44%的氨基酸同一性，分别），以人类蛋白S，在血液凝固途径的负辅助调节。通过使用抗人Gas6单特异性亲和纯化抗体，我们表明人Gas6的生物合成水平完全反映了IMR90人成纤维细胞中的mRNA表达。因此，这一发现定义了维生素K依赖性蛋白质的一个新成员，该蛋白质在许多人类和小鼠组织中表达，并可能参与与生长调节相关的蛋白酶级联反应的调节。
A set of growth arrest-specific genes (gas) whose expression is negatively regulated after serum induction has previously been described (C. Schneider, R. M. King, and L. Philipson, Cell 54:787-793, 1988). The detailed analysis of one of them, gas6, is reported here. gas6 mRNA (2.6 kb) is abundant[y expressed in serum-starved (48 h in 0.5% fetal calf serum) NIH 3T3 cells but decreases dramatically after fetal calf serum or basic fibroblast growth factor stimulation. The human homolog of gas6 was also cloned and sequenced, revealing a high degree of homology and a similar pattern of expression in IMR90 human fibroblasts. Computer analysis of the protein encoded by murine and human gas6 cDNAs showed significant homology (43 and 44% amino acid identity, respectively) to human protein S, a negative coregulator in the blood coagulation pathway. By using an anti-human Gas6 monospecific affinity-purified antibody, we show that the biosynthetic level of human Gas6 fully reflects mRNA expression in IMR90 human fibroblasts. This finding thus defines a new member of vitamin K-dependent proteins that is expressed in many human and mouse tissues and may be involved in the regulation of a protease cascade relevant in growth regulation.