Mutation of rice bc1 gene affects internode elongation and induces delayed cell wall deposition in developing internodes
Mutation of rice bc1 gene affects internode elongation and induces delayed cell wall deposition in developing internodes
复制标题
水稻bc1基因突变影响节间伸长并诱导发育节间细胞壁沉积延迟
DOI：
10.1080/15592324.2020.1749786
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发表时间：
2020
影响因子：
2.9
通讯作者：
T. Matsumoto
中科院分区：
文献类型：
--
作者：
Kanna Sato;Shin;T. Matsumoto
摘要 水稻 COBRA 样基因 BRITTLE CULM1 (BC1) 已被证明参与细胞壁成分和纤维素结晶度的组装，而纤维素结晶度决定了地上器官的机械强度。然而，人们对 BC1 在水稻发育中的详细作用知之甚少。在这项研究中，我们发现，与已知的脆秆突变体不同，bc1突变体的节间长度比水稻野生型长约1.27倍。为了分析bc1突变对节间发育的影响，我们比较了野生型、Kinmaze和bc1突变体伸长的第二节间各发育阶段细胞壁成分的沉积。在野生型中，木质素的组织化学观察表明，在节间的细胞伸长阶段之后，木质素沉积逐渐增加。纤维素和对香豆酸（pCA）含量也随着发育阶段的进展而逐渐增加。阿魏酸（FA）含量在细胞伸长阶段迅速增加，在次生细胞壁形成后期减少。 bc1突变体从细胞伸长阶段开始，细胞壁成分的含量就低于野生型，野生型中BC1在这个阶段开始表达。在bc1突变体中，细胞壁成分，尤其是木质素、pCA和FA等酚类成分的沉积模式比野生型延迟。这些结果表明，BC1 基因在节间发育的每个阶段合成适当的细胞壁方面发挥着作用。
ABSTRACT A rice COBRA-like gene, BRITTLE CULM1 (BC1) has been shown to be involved in assembling cell wall components and cellulose crystallinity, which determines mechanical strength in above ground organs. However, the detailed roles of BC1 in rice development are poorly understood. In this study, we found that, unlike the known brittle culm mutants, the internode length of the bc1 mutant was ~1.27 times longer than that of wild type in rice. In order to analyze the effects of bc1 mutation on internode development, we compared the deposition of cell wall components among each developmental stage of the elongating second internodes from wild type, Kinmaze, and the bc1 mutant. In wild type, histochemical observations of lignin revealed that lignin deposition was gradually increased after the cell elongation stage of the internodes. Cellulose and p-coumaric acid (pCA) content also gradually increased along with the progress of the developmental stage. The ferulic acid (FA) content rapidly increased in the cell elongation stage and decreased at the late secondary cell wall formation stage. In the bc1 mutant, the contents of cell wall components were lower than those of wild type from the cell elongation stage, in which the BC1 started to express at this stage in wild type. In the bc1 mutant, the deposition patterns of cell wall components, especially phenolic components including lignin, pCA, and FA, were delayed compared with those of wild type. These results suggest that the BC1 gene plays a role in synthesizing appropriate cell walls at each stage in the developing internode.
影响因子：
4.3
作者：
Sato, Kanna;Suzuki, Ryu;Katayama, Yoshihiro
通讯作者：
Katayama, Yoshihiro