Immunolocalization of ferritin in determinate and indeterminate legume root nodules
Immunolocalization of ferritin in determinate and indeterminate legume root nodules
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DOI：
10.1007/bf01282294
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发表时间：
1998-01-01
期刊：
影响因子：
2.9
通讯作者：
de Felipe, MR
中科院分区：
文献类型：
--
作者：
Lucas, MM;Van de Sype, G;de Felipe, MR
在真核生物中，铁蛋白是一种参与铁储存的蛋白质。先前已通过生化方法研究了根瘤发育早期阶段铁蛋白的出现及其与固氮活性有效性的关系。我们使用免疫细胞化学技术来确定铁蛋白的精确位置以及该蛋白质在结节发育过程中的行为。主要定位于所研究的三个豆科植物根瘤的感染和未感染细胞的质体和淀粉体中。在羽扇豆和大豆衰老根瘤的感染细胞以及不确定的苜蓿根瘤的衰老区中观察到免疫标记的减少。在大豆和羽扇豆根瘤的皮层中，铁蛋白在根瘤老化过程中增加，免疫金颗粒主要位于晶体结构中。讨论了铁蛋白和质体在结节发育过程中的假定作用。
In eukaryotic organisms ferritin is a protein involved in the storage of iron. The occurrence of ferritin and its relationship to the effectiveness of the nitrogen-fixing activity have been previously studied during the early stages of the nodule development by biochemical methods. We have used immunocytochemistry techniques to determine the precise location of ferritin and the behavior of this protein along the nodule development. The major localization was found in plastids and amyloplasts of infected and uninfected cells of the three legume nodules studied. A decrease of the immunolabelling was observed in infected cells of lupin and soybean senescing nodules and in the senescent zone of indeterminate alfalfa nodules. In the cortex of soybean and lupin nodules, ferritin increased during nodule ageing and the immunogold particles were mainly located in crystalline structures. The putative role of ferritin and plastids during nodule development is discussed.