Linkage of chondroitin-sulfate to type I collagen scaffolds stimulates the bioactivity of seeded chondrocytes in vitro
Linkage of chondroitin-sulfate to type I collagen scaffolds stimulates the bioactivity of seeded chondrocytes in vitro
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DOI：
10.1016/s0142-9612(00)00423-3
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发表时间：
2001-09-01
期刊：
影响因子：
14
通讯作者：
Veth, RPH
中科院分区：
文献类型：
--
作者：
van Susante, JLC;Pieper, J;Veth, RPH
越来越多的兴趣集中在组织工程化关节软骨植入物的潜在用途上，用于修复关节表面的缺损。从这个角度来看，各种可生物降解的支架已经被评估为将软骨细胞递送到软骨缺损中的载体。这种细胞基质植入物最终将促进创伤关节面的透明软骨再生。只有当接种的细胞在可生物降解的支架中显示出适当的增殖速率以及新的软骨特异性细胞外基质的产生时，才能用这种组织工程化软骨植入物实现成功的再生。这些代谢参数可以受到细胞递送支架的生化组成的影响。进一步阐明特定的细胞-基质相互作用对于确定用于软骨修复的细胞递送载体的最佳生化组成是重要的。在这项体外研究中，我们研究了软骨特异性糖胺聚糖在I型胶原支架中的存在对接种软骨细胞代谢活性的影响。分离的牛软骨细胞培养在多孔I型胶原基质中的存在和不存在的共价连接的硫酸软骨素（CS）长达14天。CS确实影响了接种软骨细胞的生物活性。在具有CS的I型胶原支架中，细胞增殖和保留在基质中的蛋白聚糖的总量显著更高（p < 0.001）。光学显微镜下可见基质周围有较致密的软骨层形成。扫描电子显微镜显示两种基质的初始多孔表面几乎完全表面化。各种蛋白多糖亚型的组织学和逆转录酶PCR表明，在培养过程中，接种细胞的软骨细胞表型得到了良好的保存。CS对细胞增殖和基质保留的刺激潜力，将这种GAG变成用于组织工程关节软骨的细胞递送支架的有趣的生化组分。(C)2001爱思唯尔科技有限公司版权所有。
An increasing amount of interest is focused on the potential use of tissue-engineered articular cartilage implants, for repair of defects in the joint surface. In this perspective, various biodegradable scaffolds have been evaluated as a vehicle to deliver chondrocytes into a cartilage defect. This cell-matrix implant should eventually promote regeneration of the traumatized articular joint surface with hyaline cartilage. Successful regeneration can only be achieved with such a tissue-engineered cartilage implant if the seeded cells reveal an appropriate proliferation rate in the biodegradable scaffold together with the production of a new cartilage-specific extracellular matrix. These metabolic parameters can be influenced by the biochemical composition of a cell-delivery scaffold. Further elucidation of specific cell-matrix interactions is important to define the optimal biochemical composition of a cell-delivery vehicle for cartilage repair. In this in vitro study, we investigated the effect of the presence of cartilage-specific glycosaminoglycans in a type I collagen scaffold on the metabolic activity of seeded chondrocytes. Isolated bovine chondrocytes were cultured in porous type I collagen matrices in the presence and absence of covalently attached chondroitin sulfate (CS) up to 14 days. CS did indeed influence the bioactivity of the seeded chondrocytes. Cell proliferation and the total amount of proteoglycans retained in the matrix, were significantly higher (p < 0.001) in type I collagen scaffolds with CS. Light microscopy showed the formation of a more dense cartilaginous layer at the matrix periphery. Scanning electron microscopy revealed an almost complete surfacing of the initially porous surface of both matrices. Histology and reverse transcriptase PCR for various proteoglycan subtypes suggested a good preservation of the chondrocytic phenotype of the seeded cells during culture. The stimulatory potential of CS on both the cell-proliferation and matrix retention, turns this GAG into an interesting biochemical component of a cell-delivery scaffold for use in tissue-engineering articular cartilage. (C) 2001 Elsevier Science Ltd. All rights reserved.