Vasoactive Intestinal Peptide Protects Salivary Glands against Structural Injury and Secretory Dysfunction via IL-17A and AQP5 Regulation in a Model of Sjogren Syndrome
Vasoactive Intestinal Peptide Protects Salivary Glands against Structural Injury and Secretory Dysfunction via IL-17A and AQP5 Regulation in a Model of Sjogren Syndrome
复制标题
在干燥综合征模型中，血管活性肠肽通过 IL-17A 和 AQP5 调节保护唾液腺免受结构性损伤和分泌功能障碍
DOI：
10.1159/000486859
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发表时间：
2017-01-01
影响因子：
2.4
通讯作者：
Wang, Yue
中科院分区：
文献类型：
--
作者：
Li, Chengyin;Zhua, Fenglin;Wang, Yue
目的：干燥综合征（SS）是一种累及外分泌腺的自身免疫性疾病。目前，需要既能改善异常免疫又能改善外分泌腺功能的药物。本研究旨在探讨血管活性肠肽（VIP）对SS免疫反应和外分泌腺功能的影响及其机制。研究方法：本研究采用NOD小鼠模型，观察VIP对NOD小鼠下颌下腺免疫功能和分泌功能的影响，并分析IL-17 A和AQP 5（水通道蛋白5）的表达。采用8日龄健康Sprague-Dawley大鼠的颌下腺细胞，观察VIP对AQP 5表达的影响。结果如下：我们的研究表明，VIP治疗SS小鼠模型不仅可以减轻外分泌腺的免疫损伤，而且可以改善这些腺体的分泌功能。此外，VIP显示通过下调外分泌腺中IL-17 A的表达来改善异常的免疫状态。它还通过上调AQP 5的表达来增强外分泌腺的分泌功能。结论：利用SS模型，我们发现VIP不仅可以调节免疫应答，而且可以影响外分泌腺功能，并且这些治疗作用与IL-17 A和AQP 5调节有关。(C)2018 S. Karger AG，巴塞尔
Objective: Sjogren syndrome (SS) is an autoimmune disease involving exocrine glands. Currently, drugs that can improve both abnormal immunity and exocrine gland function are needed. The study aimed to investigate the effect and mechanism of vasoactive intestinal peptide (VIP) on the immune response and exocrine gland function in SS. Methods: We investigated the effects of VIP on the immune response and secretory function of submandibular glands using NOD mice, and analyzed the expression of IL-17A and AQP5 (aquaporin 5). The submandibular gland cells from healthy 8-day-old Sprague-Dawley rats were used to observe the influence of VIP on AQP5 expression. Results: Our study shows that treatment with VIP in an SS mouse model could not only reduce the immune injury to exocrine glands but also improve the secretory function of these glands. Furthermore, VIP was shown to improve the abnormal immune status by down-regulating IL-17A expression in the exocrine glands. It also enhanced the secretory function of exocrine glands by up-regulating AQP5 expression. Conclusions: Using a model of SS, we found that VIP could not only modulate the immune response but also affect exocrine gland function, and that these therapeutic effects were associated with IL-17A and AQP5 regulation. (C) 2018 S. Karger AG, Basel