An improved model for bacterial encrustation studies.
An improved model for bacterial encrustation studies.
复制标题
细菌结壳研究的改进模型。
DOI：
10.1002/jbm.820291005
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Gage,D
中科院分区：
文献类型：
--
作者：
Sarangapani,S;Cavedon,K;Gage,D
A comparative evaluation of various biomaterials for their resistance to bacterial colonization and encrustation in infecred urine is an important area in urological biomaterials research. This article describes anin vitrodynamic perfusion system that allows four reactors containing 24 1‐in. catheter samples (6 per reactor) to be simultaneously perfused at a constant flow rate by synthetic urine. A common urease‐producing urinary pathogen,Proteus mirabilis, was maintained at a level of 106colony‐forming units/mL for 7 days in the dynamic perfusion reactors. The pH and bacterial population were monitored every 24 h and the percentage of encrustation on latex and hydrogel‐coated commercial catheter materials gave reproducible results in three different runs, 15.2 ± 3.65% and 13.8 ± 2.58%, respectively. A major issue of inlet clogging due to ascending bacteria or ammonia has been rectified using a dismountable inlet assembly. An incubator coupled with a cooling system allowed accurate temperature maintenance of 37°C in all four reactors. Results from scanning electron microscopy of some latex samples are also presented. © 1995 John Wiley & Sons, Inc.
