Half-genome human immunodeficiency virus type 1 constructs for rapid production of reporter viruses
Half-genome human immunodeficiency virus type 1 constructs for rapid production of reporter viruses
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DOI：
10.1016/s0166-0934(03)00110-1
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发表时间：
2003-06-30
影响因子：
3.1
通讯作者：
Yang, OO
中科院分区：
文献类型：
--
作者：
Ali, A;Jamieson, BD;Yang, OO
使用HIV-1分子克隆的报告构建体在研究感染对单个细胞的影响方面是有用的。本发明描述了一种产生含有报告基因的病毒半基因组构建体的策略，所述报告基因可用于产生重组完整感染性病毒体。这种方法允许从相同的基因构建体快速产生报告和非报告病毒体，避免了为待研究的每个病毒变化产生新的报告基因构建体的需要。我们提供了一个例子，该系统的效用，用于研究Nef对感染细胞的影响。(C)2003 Elsevier Science B. V.保留所有权利。
The use of reporter constructs of HIV-1 molecular clones has been useful in studying the effects of infection on individual cells. A strategy of producing viral half-genome constructs containing reporter genes that can be used to produce recombinant complete infectious virions is described. This approach allows rapid generation of reporter and non-reporter virions from the same genetic construct, obviating the need to produce a new reporter genetic construct for each viral change to be studied. We provide an example of the utility of this system for studying the effects of Nef on infected cells. (C) 2003 Elsevier Science B.V. All rights reserved.