Identification of the protein product of the Coch gene (hereditary deafness gene) as the major component of bovine inner ear protein
Identification of the protein product of the Coch gene (hereditary deafness gene) as the major component of bovine inner ear protein
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DOI：
10.1016/s0925-4439(00)00101-0
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发表时间：
2001-03-26
影响因子：
6.2
通讯作者：
Yagi, T
中科院分区：
文献类型：
--
作者：
Ikezono, T;Omori, A;Yagi, T
为了更好地了解遗传性听力障碍的原因，我们利用双向凝胶电泳法对内耳蛋白质组进行了分析。在分析过程中，我们发现了人类Coch基因产物的牛同源物非常独特的性质。Coch基因是遗传性听力障碍之一，DFNA9，最近被认为是导致梅尼埃病的一个可能的遗传因素。Coch蛋白占牛内耳蛋白的70%，由16个不同的蛋白质点组成，具有电荷和大小的异质性。这种蛋白质的异质性表明，COCH基因在转录和/或翻译后水平上有几种处理方式。关于遗传性听力障碍基因已经积累了很多知识，但关于这些基因的蛋白质产物的研究很少。这是第一份鉴定可可蛋白的报告。对Coch蛋白的研究可能会为听力和前庭疾病的发病机制提供更多信息。(C)2001 Elsevier Science B.V.保留所有权利。
In order to better understand the cause of hereditary hearing impairment, we have performed a proteomic analysis of the inner ear proteins using two-dimensional gel electrophoresis. In the process of analysis, we have found very unique properties of the bovine homologue of the human COCH gene product. The COCH gene is responsible for one of the hereditary hearing impairments, DFNA9, and was recently suggested to be a possible genetic factor contributing to Meniere's disease. The Coch protein constitutes 70% of bovine inner ear proteins and is composed of 16 different protein spots, with charge and size heterogeneity. Heterogeneity of this protein suggests that the Coch gene is processed in several ways, at the transcriptional and/or posttranslational level. Much knowledge has accumulated about the hereditary hearing impairment genes; however, little research has been done regarding the protein products of those genes. This is the first report to characterize the Coch protein. Study of the Coch protein might provide more information on the mechanism of hearing and vestibular disorders. (C) 2001 Elsevier Science B.V. All rights reserved.