Telomere-to-telomere Schizosaccharomyces japonicus genome assembly reveals hitherto unknown genome features
Telomere-to-telomere Schizosaccharomyces japonicus genome assembly reveals hitherto unknown genome features
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DOI：
10.1002/yea.3912
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发表时间：
2024-03-07
期刊：
影响因子：
2.6
通讯作者：
Nieduszynski，Conrad A.
中科院分区：
文献类型：
--
作者：
Etherington，Graham J.;Wu，Pei-Shang;Nieduszynski，Conrad A.
日本Schizosaccharomyces japonicusus属于裂糖菌单属，也被称为“裂变酵母”。作为复合模型系统的一部分，其研究广泛。pombesister物种,S。japonicus为细胞生物学机制的运作和进化提供了重要的见解。此外，其不同的生物学特性使得。日本血吸虫本身就是有价值的模式生物。然而，目前可用的基因组组装包含间隙，并且无法解决着丝粒和其他重复序列丰富的染色体区域。在这里，我们提出了一个端粒到端粒的长读基因组组装。japonicusgenome。这包括三条百万碱基长度的染色体，其着丝粒长数百千碱基，富含5S核糖体RNA基因、转移RNA基因、长末端重复序列和短重复序列。我们在2号染色体上发现了一个类似于331 kb的着丝粒重复的基因稀疏区域。我们修改了s的基因组大小。日本至少有16.6 Mb，可能达到18.12 Mb，比以前的估计至少大30%。我们的整个基因组组合将支持生长。研究方向包括着丝粒和DNA重复进化、酵母比较基因组学等。
Schizosaccharomyces japonicusbelongs to the single‐genus class Schizosaccharomycetes, otherwise known as “fission yeasts.” As part of a composite model system with its widely studiedS. pombesister species,S. japonicushas provided critical insights into the workings and the evolution of cell biological mechanisms. Furthermore, its divergent biology makesS. japonicusa valuable model organism in its own right. However, the currently available genome assembly contains gaps and has been unable to resolve centromeres and other repeat‐rich chromosomal regions. Here we present a telomere‐to‐telomere long‐read genome assembly of theS. japonicusgenome. This includes the three megabase‐length chromosomes, with centromeres hundreds of kilobases long, rich in 5S ribosomal RNA genes, transfer RNA genes, long terminal repeats, and short repeats. We identify a gene‐sparse region on chromosome 2 that resembles a 331 kb centromeric duplication. We revise the genome size ofS. japonicusto at least 16.6 Mb and possibly up to 18.12 Mb, at least 30% larger than previous estimates. Our whole genome assembly will support the growingS. japonicusresearch community and facilitate research in new directions, including centromere and DNA repeat evolution, and yeast comparative genomics.