THE MODULATION OF THE CONVERSION OF 1-AMINOCYCLOPROPANE-1-CARBOXYLIC ACID TO ETHYLENE BY LIGHT
THE MODULATION OF THE CONVERSION OF 1-AMINOCYCLOPROPANE-1-CARBOXYLIC ACID TO ETHYLENE BY LIGHT
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DOI：
10.1007/bf00383887
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发表时间：
1981-01-01
期刊：
影响因子：
4.3
通讯作者：
VANLOON, LC
中科院分区：
文献类型：
--
作者：
DELAAT, AMM;BRANDENBURG, DCC;VANLOON, LC
光照和黑暗条件下烟叶内源乙烯的产生量基本相同。然而，外源施加的1-氨基环丙烷-1-羧酸（ACC）转化为乙烯的速率被可逆地抑制光。在烟草叶片对烟草花叶病毒的过敏反应中，病毒刺激的乙烯产生同样受到光的抑制。在这种情况下，乙烯生产被限制在ACC转化为乙烯的水平。放线菌酮和2-（4-甲基-2，6-二硝基苯胺基）-N-甲基-丙酰胺在ACC刺激的乙烯产生增加的抑制后，将叶盘从光转移到黑暗中，表明从头蛋白质降解参与。可以排除Gepstein和Thimann（1980，Planta 149，196-199）提出的通过必需SH基团的可逆氧化/还原调节ACC依赖性乙烯产生。相反，建议在合成/降解和活化/失活水平上调节ACC转化酶。光敏色素不参与光抑制，但低强度的红光或蓝光降低ACC转化率。二氯苯基二甲基脲抵消了光的抑制作用，表明（部分）光合系统参与了光抑制。乙烯生产ofPharbitiscotyledons生长在黑暗或光照下，无论是在存在或不存在的抑制剂类胡萝卜素合成，SAN 9789（氟草松），支持这一观点。
Endogenous ethylene production of tobacco leaves was similar in light and in darkness. However, the rate of conversion of exogenously applied l-aminocyclopropane-l-carboxylic acid (ACC) to ethylene was reversibly inhibited by light. Virus-stimulated ethylene production, during the hypersensitive reaction of tobacco leaves to tobacco mosaic virus, was likewise inhibited by light. Under such circumstances ethylene production is limited at the level of the conversion of ACC to ethylene. Inhibition of the increase in ACC-stimulated ethylene production by cycloheximide and 2-(4-methyl-2,6-dinitroanilino)-N-methyl-propionamide after shifting leaf discs from light to darkness indicated that de novo protein synthsis was involved. Regulation of ACC-dependent ethylene production by reversible oxidation/reduction of essential SH groups, as suggested by Gepstein and Thimann (1980, Planta149, 196–199) could be excluded. Instead, regulation of the ACC-converting enzyme at the level of both synthesis/degradation and activation/inactivation is suggested. Phytochrome was not involved in light inhibition, but low intensities of either red or blue light decreased the rate of ACC conversion. Dichlorophenyldimethylurea counteracted the inhibitory effect of light, indicating that (part of) the photosynthetic system is involved in the light inhibition. The ethylene production ofPharbitiscotyledons grown in darkness or light, either in the presence of absence of the inhibitor of carotenoid synthesis, SAN 9789 (norflurazon), supported this view.