Nerve growth factor expression and release in allergic inflammatory disease of the upper airways.
Nerve growth factor expression and release in allergic inflammatory disease of the upper airways.
复制标题
上呼吸道过敏性炎症性疾病中神经生长因子的表达和释放。
DOI：
10.1164/ajrccm.161.5.9908028
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Togias,A
中科院分区：
文献类型：
--
作者：
Sanico,AM;Stanisz,AM;Gleeson,TD;Bora,S;Proud,D;Bienenstock,J;Koliatsos,VE;Togias,A
众所周知，过敏性气道疾病的特征是炎症和高反应性，但这两种情况之间的联系尚未阐明。我们以前已经表明，在过敏性鼻炎，高反应性是由于增加神经反应。因此，我们假设，神经生长因子（NGF），这是表达的炎症细胞和影响的变化，导致增加神经反应，可能是一个关键的介质在这种疾病。使用逆转录-聚合酶链反应（RT-PCR），Western免疫印迹和ELISA来评估NGF的表达和释放，我们发现，与对照组相比，变应性鼻炎患者的浅表鼻刮片中NGF mRNA显著降低，鼻灌洗液中NGF蛋白的基线浓度显著升高。过敏原鼻腔激发显著增加鼻灌洗液中的神经生长因子蛋白的过敏性鼻炎的主题，但不是对照组。鼻灌洗液中NGF蛋白的浓度不受变应原或组胺刺激的影响。这些数据提供了在变应性鼻炎中粘膜NGF表达和释放的稳态失调的第一个证据，并支持这种神经营养因子在人类气道变应性炎性疾病的病理生理学中的作用。
It is well known that allergic airways disease is characterized by inflammation and hyperresponsiveness, but the link between these two conditions has not been elucidated. We have previously shown that in allergic rhinitis, hyperresponsiveness is attributable to increased neural reactivity. We thus hypothesized that nerve growth factor (NGF), which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in this disease. Using reverse transcription–polymerase chain reaction (RT-PCR), Western immunoblotting, and ELISA to evaluate NGF expression and release, we found that subjects with allergic rhinitis have significantly decreased NGF mRNA in superficial nasal scrapings and significantly higher baseline concentrations of NGF protein in nasal lavage fluids, compared with control subjects. Nasal provocation with allergen significantly increased NGF protein in nasal lavage fluids of subjects with allergic rhinitis, but not of control subjects. The concentrations of NGF protein in nasal lavage fluids were not affected by provocation with the vehicle for allergen or with histamine. These data provide the first evidence of a steady state of dysregulation in mucosal NGF expression and release in allergic rhinitis, and support a role of this neurotrophin in the pathophysiology of allergic inflammatory disease of the human airways.