The chondrogenic potential of periosteum decreases with age
The chondrogenic potential of periosteum decreases with age
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DOI：
10.1016/s0736-0266(00)00014-0
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发表时间：
2001-01-01
影响因子：
2.8
通讯作者：
Fitzimmons, JS
中科院分区：
文献类型：
--
作者：
O'Driscoll, SWM;Saris, DBF;Fitzimmons, JS
骨膜含有未分化的间充质干细胞，在软骨修复和骨折愈合过程中具有成软骨的潜力。随着年龄的增长，骨膜的成软骨潜能明显下降。采用器官培养模型研究骨膜成软骨潜能与衰老的关系。采用琼脂糖悬浮液培养82只2周龄~ 2岁家兔胫骨近内侧段736块骨膜外植体，采用组织形态学、胶原分型、湿重测定、h -3胸腺嘧啶和s -35-硫酸根摄取、放射自显影和PCNA免疫染色等方法进行软骨形成分析。6个月时骨骼发育成熟，12个月时体重停止增长。随着年龄的增长，软骨形成显著减少（P < 0.0001），在1.5 ~ 2月龄时软骨形成最大。6月龄兔的软骨形成减少50%，到12月龄时软骨形成达到稳定的最低水平。在软骨形成电位下降的同时，h -3-胸腺嘧啶的摄取（P < 0.0001）、s -35-硫酸盐的摄取（P = 0.0117）、骨膜形成层的厚度（P < 0.0001）和细胞总数（P < 0.0001）也出现了类似的下降。h -3胸腺嘧啶放射自显像和PCNA免疫染色证实了软骨细胞前体所在的形成层的增殖活性下降，尽管增殖细胞的百分比没有随着年龄的增长而显着变化。变化最显著的是2 ~ 12月龄家兔包皮形成层厚度和总细胞数显著减少（87%）（P < 0.05）。这些数据证实骨膜的成软骨潜能随着年龄的增长而下降。因此，在骨骼成熟后通过骨膜移植改善软骨形成的一种可能性是在软骨形成早期刺激软骨细胞前体池中细胞总数的增加。2001骨科研究学会。Elsevier Science Ltd.出版。版权所有。
Periosteum contains undifferentiated mesenchymal stem cells that possess the potential for chondrogenesis during cartilage repair and in fracture healing. With aging, the chondrogenic potential of periosteum declines significantly. An organ-culture model was used to investigate the relationship between the chondrogenic potential of periosteum and aging. A total of 736 periosteal explants from the proximal medial tibiae of 82 rabbits, aged 2 weeks to 2 years, were cultured in agarose suspension conditions conductive for chondrogenesis, and analyzed using histomorphometry, collagen typing, wet weight measurement, H-3-thymidine and S-35-sulfate uptake, autoradiography and PCNA immunostaining. The rabbits were skeletally mature by 6 months and stopped increasing in weight bq 12 months. Chondrogenesis declined significantly with age (P < 0.0001) and was maximal in the 1.5-2 month-old rabbits. Explants from the 6 month-old rabbits formed 50% less cartilage, and by 12 months chondrogenesis reached a steady state minimal level. In parallel with this decrease in chondrogenic potential similar decreases were measured in H-3-thymidine uptake (P < 0.0001), S-35-sulfate uptake (P = 0.0117), as well as the thickness (P < 0.0001) and the total number of cells in the cambium layer of the periosteum (P < 0.0001). Autoradiography with H-3-thymidine and PCNA immunostaining confirmed the measured decrease in proliferative activity in the cambium layer where the chondrocyte precursors reside, although the percentage of proliferating cells did not change significantly with age. The most dramatic change was the marked decrease (87%) in the thickness and total cell number in the cambium layer of the perisoteum between the 2 and 12 month-old rabbits (P < 0.05). These data confirm a decline in the chondrogenic potential of periosteum with aging. Thus, one possibility for improving cartilage formation by periosteal transplantation after skeletal maturity would be to stimulate an increase in the total number of cells in the chondrocyte precursor pool early during chondrogenesis. 2001 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.