REGIONAL CHANGES IN BRAIN OXYTOCIN RECEPTORS POSTPARTUM - TIME-COURSE AND RELATIONSHIP TO MATERNAL-BEHAVIOR
REGIONAL CHANGES IN BRAIN OXYTOCIN RECEPTORS POSTPARTUM - TIME-COURSE AND RELATIONSHIP TO MATERNAL-BEHAVIOR
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DOI：
10.1111/j.1365-2826.1990.tb00445.x
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发表时间：
1990-01-01
影响因子：
3.2
通讯作者：
INSEL, TR
中科院分区：
文献类型：
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作者：
INSEL, TR
在之前的一份报告中，大鼠大脑中的催产素受体在产后早期增加。目前的研究开始使用新型的、高选择性的催产素受体配体[125I]d(CH2)5[Tyr(Me)2, Thr4, Tyr- nh29]OVT （[125I]-OTA）来复制这一发现。在妊娠第15天、产后第1天和第6天以及哺乳期结束后至少6天，采用体外受体放射自显影法测定大鼠脑内的结合情况。与怀孕相比，产后第1天（而不是第6天），尾纹床核和下丘脑腹内侧核的催产素受体结合增加。在其他几个区域，包括前嗅核、杏仁核中央核和腹侧耻骨下捆绑，在两组之间是相同的。最令人惊讶的是，哺乳后组的终纹床核结合率最低（产后第1天组比哺乳后组高87%）。饱和度研究表明，结合差异反映的是位点数量的变化，而不是亲和力的变化。这些发现与早期关于外源性性腺类固醇给药后脑催产素受体变化的研究一致。为了确定由非激素手段（即凹形）引起的母性行为是否也与催产素受体增加有关，研究了大量接触幼犬的处女雌性。在未怀孕的母性雌性中，尾纹床核的催产素受体结合没有变化，这表明产后观察到的增加对母性行为的开始不是必需的。
In a previous report, receptors for oxytocin in rat brain were reported to increase during the early post-partum period. The current study set out to replicate this finding using the novel, highly selective oxytocin receptors ligand, [125I]d(CH2)5[Tyr(Me)2, Thr4, Tyr-NH29]OVT ([125I]-OTA). Binding was measured using in vitro receptor autoradiography in rat brain on Day 15 of pregnancy, on Days 1 and 6 post-partum, and at least 6 days following the end of lactation. Relative to pregnancy, oxytocin receptor binding was increased on Day 1 (but not Day 6) post-partum in the bed nucleus of the stria terminalis and the ventromedial nucleus of the hypothalamus. In several other regions, including the anterior olfactory nucleus, the central nucleus of the amygdala, and the ventral subiculum binding was equivalent across the groups. Most surprising, binding in the bed nucleus of the stria terminalis was lowest in the post-lactating group (post-partum Day 1 group was 87% higher than post-lactating group). Saturation studies suggested that binding differences reflected changes in number and not affinity of sites. These findings are consistent with earlier studies of changes in brain oxytocin receptors following exogenous gonadal steroid administration. To determine if maternal behaviour elicited by nonhormonal means (i.e. concaveation) was also associated with increased oxytocin receptors, virgin females with extensive pup exposure were studied. No change in oxytocin receptor binding in the bed nucleus of the stria terminalis was noted in virgin, maternal females-demonstrating that the increases observed post-partum was not essential for the onset of maternal behaviour.