Real-time PET analysis of metastatic tumor cell trafficking in vivo and its relation to adhesion properties.
Real-time PET analysis of metastatic tumor cell trafficking in vivo and its relation to adhesion properties.
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体内转移性肿瘤细胞运输的实时 PET 分析及其与粘附特性的关系。
DOI：
10.1016/0005-2736(95)00123-k
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
S. Okada
中科院分区：
文献类型：
--
作者：
C. Koike;N. Oku;M. Watanabe;H. Tsukada;T. Kakiuchi;T. Irimura;S. Okada
Although a number of studies have indicated that highly metastatic cells tend to adhere more to target endothelium in vitro than low or non-metastatic cells, direct evidence about the correlation between cellular adhesiveness and organ disposition of the cells has not been obtained. Using positron emission tomography (PET), we have developed a novel technique that enables the non-invasive detection of the real-time tumor cell trafficking. The present study shows the correlation between trafficking of murine large cell lymphoma RAW117 and the adhesion properties of the cells in vitro. Cells accumulated in the liver time-dependently, and accumulation of RAW117-H10, liver metastatic subline cells, was more intense than that of RAW117-P, the parental cells, indicating that the metastatic potential is correlated with the in vivo accumulation of the cells in the target tissue. To examine whether the adhesion properties of the cell membrane determine the cell trafficking, we performed PET analysis after altering the adhesion properties on the cell membrane by means of cellular protein kinase C modulation, since the modulation of this enzyme is known to alter the surface adhesion molecules, i.e., those of the integrin superfamily. The treatment of RAW117-P with 12-O-tetradecanoylphorbol 13-acetate, which caused augmentation of adhesion to hepatic sinusoidal microvessel endothelial cells (HSE) in vitro, enhanced the hepatic accumulation of the cells in vivo. On the contrary, treatment of RAW117-H10 with the protein kinase C inhibitor H-7, 1-(5-isoquinolinesulfonyl)-2-methylpiperazine dihydrochloride, which reduced the adhesion activity of the cells to HSE, suppressed their accumulation in the liver, although the suppression was observed only during the first 30 min after administration of the cells. These data suggest that the adhesion properties of metastatic lymphoma cells are critical for the accumulation of these cells in the target tissue.
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鼠大细胞淋巴瘤选定转移变体的粘附、侵袭和生长特性。
DOI：
--
发表时间：
1989
期刊：
Invasion & metastasis
影响因子：
--
作者：
Nicolson,GL;Belloni,PN;Tressler,RJ;Dulski,K;Inoue,T;Cavanaugh,PG
通讯作者：
Cavanaugh,PG
糖蛋白 IIIa 在完整的人血小板中被磷酸化。
影响因子：
20.3
作者：
Parise,LV;Criss,AB;Nannizzi,L;Wardell,MR
通讯作者：
Wardell,MR
影响因子：
56.9
作者：
RICE, GE;BEVILACQUA, MP
通讯作者：
BEVILACQUA, MP
病毒重复感染对小鼠 RAW117 大细胞淋巴瘤的细胞表面糖蛋白、巨噬细胞细胞抑制和血源性转移特性的修饰。
影响因子：
11.2
作者：
Yoshida,M;Gallick,GE;Irimura,T;Nicolson,GL
通讯作者：
Nicolson,GL
原发性和转移性黑色素瘤病变中细胞间粘附分子1的差异表达。
影响因子：
11.2
作者：
Natali,P;Nicotra,MR;Cavaliere,R;Bigotti,A;Romano,G;Temponi,M;Ferrone,S
通讯作者：
Ferrone,S