Retinal ganglion cell degeneration is topological but not cell type specific in DBA/2J mice.
Retinal ganglion cell degeneration is topological but not cell type specific in DBA/2J mice.
复制标题
视网膜神经节细胞变性是拓扑的，但在DBA/2J小鼠中不是特异性细胞类型。
DOI：
10.1083/jcb.200506099
复制
发表时间：
2005-10-24
影响因子：
7.8
通讯作者：
Masland, Richard H
中科院分区：
文献类型：
--
作者：
Jakobs, Tatjana C;Libby, Richard T;Ben, Yixin;John, Simon W M;Masland, Richard H
利用多种双重和三重标记技术，我们重新评估了遗传性青光眼小鼠模型中视网膜神经元的死亡。细胞特异性标记物和总神经元计数显示，除神经节细胞外，任何视网膜神经元均无细胞丢失。在我们定义细胞类型的能力范围内，没有一组神经节细胞特别容易受到或抵抗退化。逆行标记和神经丝染色显示，在该青光眼模型中，轴突萎缩、树突重塑和体细胞收缩（至少是最大的细胞类型）先于神经节细胞死亡。细胞死亡或存活的区域从视神经乳头呈扇形放射状分布。总的来说，这些数据表明视神经乳头的轴突损伤是早期病变，轴突束的损伤会导致这种退化模式。然而，小鼠眼睛的结构似乎排除了通常假设的神经头内的机械损伤源。
Using a variety of double and triple labeling techniques, we have reevaluated the death of retinal neurons in a mouse model of hereditary glaucoma. Cell-specific markers and total neuron counts revealed no cell loss in any retinal neurons other than the ganglion cells. Within the limits of our ability to define cell types, no group of ganglion cells was especially vulnerable or resistant to degeneration. Retrograde labeling and neurofilament staining showed that axonal atrophy, dendritic remodeling, and somal shrinkage (at least of the largest cell types) precedes ganglion cell death in this glaucoma model. Regions of cell death or survival radiated from the optic nerve head in fan-shaped sectors. Collectively, the data suggest axon damage at the optic nerve head as an early lesion, and damage to axon bundles would cause this pattern of degeneration. However, the architecture of the mouse eye seems to preclude a commonly postulated source of mechanical damage within the nerve head.