Mu- and delta-opioid receptor binding peaks and kappa-homogeneity in the molecular layers of rat hippocampal formation.
Mu- and delta-opioid receptor binding peaks and kappa-homogeneity in the molecular layers of rat hippocampal formation.
复制标题
大鼠海马结构分子层中的 Mu 和 δ 阿片受体结合峰和 kappa 均质性。
DOI：
10.1016/0006-8993(88)91215-2
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发表时间：
1988
期刊：
影响因子：
2.9
通讯作者：
Vogt,BA
中科院分区：
文献类型：
--
作者：
Plager,MD;Vogt,BA
本文研究了（d-Ala-NMe-Phe-Gly-ol）-脑啡肽（DAGO）、（2-d-青霉胺，5-d-青霉胺）-脑啡肽（DPDPE）和强啡肽A（1-8）（DYN）在大鼠海马结构CA_1区和齿状回的层下结合模式。使用盖玻片放射自显影和单颗粒计数技术在冷冻切片机切片上测定结合。DAGO是μ-位点激动剂，在放射层远端有一个第二峰，在放射层远端有一个峰。DAGO在齿状回的结合是均匀的。δ-位点激动剂DPDPE在海马神经层也有结合峰，但在海马分子层没有第二峰。在齿状回，DPDPE结合是最高的内部三分之一的分子层紧邻颗粒细胞层。内源性阿片DYN具有模仿DAGO的层状结合特征。然而，当氚代DYN与未标记DAGO和DPDPE共孵育时，多达90%的DYN结合被阻断，剩余的结合是均匀的，尽管在皮层中保留了一个小峰。辐射层中DAGO结合的第二峰对应于先前确定的包含脑啡肽和γ-氨基丁酸免疫反应性过程的区域。这种对应关系表明，阿片类化合物可能介导的海马锥体神经元的远端树突的去抑制。此外，DYN结合模式表明，其在大鼠海马中的作用可能是通过μ-和δ-受体。
The sublaminar binding profiles of (d-Ala-NMe-Phe-Gly-ol)-enkephalin (DAGO), (2-d-penicillamine, 5-d-penicillamine)-enkephalin (DPDPE), and dynorphin A(1–8) (DYN) were studied in the CA1 subfield and dentate gyrus of rat hippocampal formation. Binding was assayed on cryomicrotome sections using coverslip autoradiographic and single grain counting techniques. DAGO, an agonist for μ-sites, had peak binding in the stratum pyramidale with a secondary peak in the distal part of the stratum radiatum. Binding of DAGO in the dentate gyrus was homogeneous. DPDPE, a δ-site agonist, also had peak binding in the stratum pyramidale, but there was no secondary peak in the molecular layer of the hippocampus. In the dentate gyrus, DPDPE binding was highest in the inner one-third of the molecular layer immediately adjacent to the granular cell layer. The endogenous opioid DYN had a laminar binding profile that mimicked that of DAGO. However, when tritiated DYN was coincubated with unlabeled DAGO and DPDPE, as much as 90% of DYN binding was blocked and remaining binding was homogenous though a small peak remained in the stratum pyramidale. The secondary peak of DAGO binding in the stratum radiatum corresponds to an area previously determined to contain processes immunoreactive for enkephalin and gamma aminobutyric acid. This correspondence suggests that opioid compounds may mediate disinhibition of the distal dendrites of hippocampal pyramidal neurons. In addition, DYN binding patterns indicate that its action in rat hippocampus is likely by both μ- and δ-receptors.