Prevalence of Borna disease virus antibodies in healthy Japanese black cattle in Kyushu
Prevalence of Borna disease virus antibodies in healthy Japanese black cattle in Kyushu
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DOI：
10.1292/jvms.68.171
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发表时间：
2006-02-01
影响因子：
1.2
通讯作者：
Tomonaga, K
中科院分区：
文献类型：
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作者：
Watanabe, Y;Yanai, H;Tomonaga, K
流行病学研究表明，日本多种动物中均发现博尔纳病病毒（BDV）无症状感染。最近的报告还显示，该病毒引起的神经系统疾病可能存在于该国的马、牛、狗和猫身上。在这项研究中，我们使用 ELISA 和蛋白质免疫印迹法调查了在日本最南端主岛九州饲养的日本黑牛中 BDV 抗体的血清阳性率。在 101 份血清样本中，分别鉴定出 11 份（10.9%）和 21 份（20.7%）血清含有 BDV N 和 P 抗原抗体。在阳性血清中，三头奶牛（2.9%）的两种抗原均呈血清阳性。此外，有趣的是，只有雌性奶牛表现出 P 抗体，而雄性和雌性奶牛中都检测到了 N 抗体，比例相当。结合之前的研究，我们的结果表明 BDV 可能在日本饲养的牛中广泛传播。此外，这是第一份报告表明肉牛（日本黑牛）具有针对可能的人畜共患病原体 BDV 的抗体。
Epidemiological studies have demonstrated that asymptomatic infection of Borna disease virus (BDV) is found in various species of animals in Japan. Recent reports have also revealed that neurological diseases caused by this virus could exist in horses, cattle, a dog, and cats in this country. In this study, we investigated seroprevalence of BDV antibodies in Japanese black cows reared in Kyushu, the southernmost main island of Japan, using ELISA and Western-immunoblotting. Of 101 serum samples, 11 (10.9%) and 21(20.7%) sera were identified as having antibodies to the BDV N and P antigens, respectively. Among the positive sera, three cows (2.9%) were seropositive for both of the antigens. Furthermore, interestingly, only female cows showed antibodies to P, whereas N antibodies were detected in male and female cows with a comparative ratio. Together with previous studies, our results indicate that BDV might be widely spread in cattle raised in Japan. Furthermore, this is the first report to show that beef cattle, Japanese black cattle, have antibodies against a possible zoonotic pathogen, BDV.