Cell traffic between donor and recipient following rat limb allograft
Cell traffic between donor and recipient following rat limb allograft
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DOI：
10.1016/j.orthres.2004.06.009
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发表时间：
2005-01-01
影响因子：
2.8
通讯作者：
Doi, K
中科院分区：
文献类型：
--
作者：
Muramatsu, K;Kurokawa, Y;Doi, K
虽然在同种异体器官移植中已经注意到移植物进入受体和受体进入移植物的细胞运输，但在全肢移植中却知之甚少。本研究是为了确定细胞在供体和受体之间的迁移。对67只性别不匹配的大鼠进行了带血管的后肢异体移植，并对受体动物进行了FK506免疫抑制。利用y染色体特异性引物，采用半定量PCR法测定供体细胞和受体细胞的比例。同种异体移植肢体在最后评估前没有出现排斥反应。男性受体细胞不是在移植后1周，而是在移植后48周在女性肢体移植物中检测到。在女性受体的肱骨和胫骨中检测到男性供体细胞，但在腓肠肌和腿部皮肤中未检测到。我们的结果表明，受体来源的细胞逐渐迁移到移植骨中。肌肉和皮肤细胞随时间的推移而变化。供体来源的细胞迁移到健康的骨骼，但没有迁移到健康的肌肉和皮肤。由于主动再生发生在移植物肢体中，以补偿因缺血和手术干预引起的移植物损伤，受体来源的细胞可能介导肌肉和真皮表皮的更新。(C) 2004骨科研究学会。Elsevier Ltd.出版。版权所有。
Although cell traffic from the graft into the recipient and from the recipient into the graft had been noticed in allogeneic organ transplantation, little is known following whole-limb allografting. This study was conducted to define cell migration between donor and recipient. Sixty-seven vascularized hind limb allotransplantations were performed in rat sex-mismatched pairs and the recipient animals were treated with FK506 inimunosuppression. The ratio of donor and recipient cells was evaluated by semi-quantitative PCR using the specific primers of the Y-chromosome. Allografted limbs had no rejection episode until the final assessment. The male recipient cells were detected in female limb grafts not at 1 week but at 48 weeks after transplantation. The male donor cells were detected in the humerus and tibia in the female recipient but not in the gastrocnemius muscle and leg skin. Our results demonstrated that recipient-derived cells gradually migrated into the grafted bone. muscle and skin cells with the duration of time. Donor-derived cells migrated into the healthy bones but not into the healthy muscle and skin. Because active regeneration occurs in the grafted limb to compensate graft damage secondary to ischemia and operative intervention, recipient-derived cells may mediate a muscular and dermo-epidermal renewal. (C) 2004 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved.