Arabidopsis proteins with a transposon-related domain act in gene silencing.
Arabidopsis proteins with a transposon-related domain act in gene silencing.
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DOI：
10.1038/ncomms15122
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发表时间：
2017-05-03
影响因子：
16.6
通讯作者：
Mathieu O
中科院分区：
文献类型：
--
作者：
Ikeda Y;Pélissier T;Bourguet P;Becker C;Pouch-Pélissier MN;Pogorelcnik R;Weingartner M;Weigel D;Deragon JM;Mathieu O
转座因子（TE）在大多数真核生物中普遍存在，宿主基因组设计了沉默策略来控制TE活性。其中之一，转录沉默，通常与DNA甲基化和短干扰RNA有关。在这里，我们表明，拟南芥基因MAIL1和MAIN定义了一个替代沉默途径，独立于DNA甲基化和短干扰RNA。MAIL1或MAIN的突变体表现出沉默的释放，并且似乎表现出着丝粒周围异染色质的凝聚受损。系统发育分析表明，MAIL 1和MAIN不仅编码一个与反转录转座子相关的植物移动的结构域，而且在植物进化过程中，宿主植物的移动的结构域被DNA转座子捕获。我们的研究结果揭示了转座子相关结构域的拟南芥蛋白在基因沉默中的作用。宿主基因组已经进化出多种沉默机制来抑制转座因子的活性。Ikeda等人在此表明，拟南芥MAIL 1和MAIN基因编码一个与反转录转座子相关的植物移动的结构域，并定义了一个在很大程度上独立于DNA甲基化和siRNA的替代沉默途径。
Transposable elements (TEs) are prevalent in most eukaryotes, and host genomes have devised silencing strategies to rein in TE activity. One of these, transcriptional silencing, is generally associated with DNA methylation and short interfering RNAs. Here we show that the Arabidopsis genes MAIL1 and MAIN define an alternative silencing pathway independent of DNA methylation and short interfering RNAs. Mutants for MAIL1 or MAIN exhibit release of silencing and appear to show impaired condensation of pericentromeric heterochromatin. Phylogenetic analysis suggests not only that MAIL1 and MAIN encode a retrotransposon-related plant mobile domain, but also that host plant mobile domains were captured by DNA transposons during plant evolution. Our results reveal a role for Arabidopsis proteins with a transposon-related domain in gene silencing. Host genomes have evolved multiple silencing mechanisms to repress transposable element activity. Here Ikeda et al. show that the Arabidopsis MAIL1 and MAIN genes encode a retrotransposon-related plant mobile domain and define an alternative silencing pathway largely independent of DNA methylation and siRNAs.