Polymer Based Gene Silencing: In Vitro Delivery of SiRNA.
Polymer Based Gene Silencing: In Vitro Delivery of SiRNA.
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基于聚合物的基因沉默：SiRNA 的体外递送。
DOI：
10.1007/978-1-4939-3718-9_9
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Carlos MI
中科院分区：
文献类型：
--
作者：
Carlos MI
基因沉默可以通过利用RNA干扰机制来实现蛋白质表达的下调。SiRNA的治疗作用依赖于它对细胞内间隙的输送。本章介绍了siRNA Byn-(2-乙氨基)-6-O-糖基壳聚糖(EAGC)的传递。EAGC是一种以壳聚糖为基础的聚合物，它与siRNA结合形成纳米颗粒(NPs)。本章以EAGC为例介绍了确定聚合物输送能力所需的步骤。步骤包括：将EAGC-siRNA多聚体导入细胞，用Western blotting检测蛋白质。
Gene silencing may be achieved by harnessing the RNA interference mechanism to effect down-regulation of protein expression. The therapeutic use of siRNA is dependent on its delivery to the intracellular space. This chapter describes the delivery of siRNA byN-(2-ethylamino)-6-O-glycolchitosan (EAGC). EAGC is a chitosan-based polymer, which binds to siRNA to form nanoparticles (NPs). The steps necessary to determine the delivery capacity of a polymer are presented in this chapter using EAGC as an example. The steps include: the transfection of cells with EAGC-siRNA polyplexes and protein detection by a Western Blotting assay.