A microfabricated fluidic device for performing two-dimensional liquid-phase separations
A microfabricated fluidic device for performing two-dimensional liquid-phase separations
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DOI：
10.1021/ac000578r
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发表时间：
2000-11-01
影响因子：
7.4
通讯作者：
Ramsey, JM
中科院分区：
文献类型：
--
作者：
Rocklin, RD;Ramsey, RS;Ramsey, JM
设计并演示了一种结合胶束电动色谱和高速开通道电泳在单一结构上的微制造流体装置，用于肽的快速自动二维分析。微芯片通过从第一维快速采样和分析第二维的流出物来工作。每隔几秒钟进行和完成第二次维分析，对色氨酸的总分析时间小于10分钟。二维分离的峰值容量估计在500-1000范围内。分离技术的正交性是最大化峰值容量或分辨率元素的重要因素，通过独立检查每种技术进行肽分离来验证。发现二维分离策略比单独在任何一个维度上获得的解析能力都要大。
A microfabricated fluidic device that combines micellar electrokinetic chromatography and high-speed open-channel electrophoresis on a single structure for the rapid automated two-dimensional analysis of peptides has been devised and demonstrated. The microchip operates by rapidly sampling and analyzing effluent in the second dimension from the first dimension. Second-dimension analyses are performed and completed every few seconds, with total analysis times of less than 10 min for tryptic peptides. The peak capacity of the two-dimensional separations has been estimated to be in the 500-1000 range. The orthogonality of the separation techniques, an important factor for maximizing peak capacity or resolution elements, was verified by examining each technique independently for peptide separations. The two-dimensional separation strategy was found to greedy increase the resolving power over that obtained for either dimension alone.