Precursor processing for plant peptide hormone maturation by subtilisin-like serine proteinases
Precursor processing for plant peptide hormone maturation by subtilisin-like serine proteinases
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DOI：
10.1126/science.aai8550
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发表时间：
2016-12-23
期刊：
影响因子：
56.9
通讯作者：
Schaller, Andreas
中科院分区：
文献类型：
--
作者：
Schardon, Katharina;Hohl, Mathias;Schaller, Andreas
调节植物生长和发育的肽激素是通过蛋白水解加工从较大的前体蛋白衍生而来的。我们的研究解决了枯草杆菌蛋白酶样蛋白酶（SBT）在这一过程中的作用。使用组织特异性表达的蛋白酶抑制剂作为一种工具，以克服功能冗余，我们发现，SBT活性所需的IDA（开花缺乏脱落），在拟南芥花器官的脱落肽信号的成熟。我们确定了三种体外处理IDA前体的SBT，并且这种处理被证明是体内形成作为内源性信号肽的mIDA（IDA的成熟和生物活性形式）所需的。因此，SBT在植物中充当激素原转化酶，并且几个功能冗余的SBT有助于信号生物合成。
Peptide hormones that regulate plant growth and development are derived from larger precursor proteins by proteolytic processing. Our study addressed the role of subtilisin-like proteinases (SBTs) in this process. Using tissue-specific expression of proteinase inhibitors as a tool to overcome functional redundancy, we found that SBT activity was required for the maturation of IDA (INFLORESCENCE DEFICIENT IN ABSCISSION), a peptide signal for the abscission of floral organs in Arabidopsis. We identified three SBTs that process the IDA precursor in vitro, and this processing was shown to be required for the formation of mIDA (the mature and bioactive form of IDA) as the endogenous signaling peptide in vivo. Hence, SBTs act as prohormone convertases in plants, and several functionally redundant SBTs contribute to signal biogenesis.