Transformation of human glioma cell lines with the p16 gene inhibits cell proliferation.
Transformation of human glioma cell lines with the p16 gene inhibits cell proliferation.
复制标题
用 p16 基因转化人神经胶质瘤细胞系可抑制细胞增殖。
作者：
S. Hama;T. Sadatomo;H. Yoshioka;K. Kurisu;E. Tahara;I. Naruse;Y. Heike;N. Saijo
我们用Southern杂交的方法检测了5个人脑胶质瘤细胞系中p16基因的基因组状态。P16基因位于9p21染色体区域，在5个人脑胶质瘤细胞系中有4个(80%)和15个临床标本中有5个(33%)检测到纯合子缺失。以pRc/CMV-p16为载体，将人p16全长基因导入p16缺失的人脑胶质瘤细胞系U251 MG细胞，观察p16基因转移对恶性胶质瘤细胞生长的抑制作用。P16基因的导入通过使U251 MG细胞周期停滞于G1期而抑制细胞生长。我们还检测了p16基因转移对顺铂、1-(4-amino-2-methyl-5-pyrimidinyl)methyl-3-(2-chloroethyl)-3-亚硝脲盐酸盐和5‘-氮胞苷化疗敏感性的影响。我们没有检测到p16基因转移后它们的任何变化。这些结果可能提示p16基因缺失促进了人脑胶质瘤的无限制生长，但与其对CDDP、ACNU和AZC的化疗敏感性无关。
We examined the genomic status of the p16 gene in 5 human glioma cell lines by Southern blot analysis. The p16 gene was located in the 9p21 chromosomal region and homozygous deletion was detected in 4 of 5 (80%) human glioma cell lines and 5 of 15 (33%) clinical samples. We transfected the full-length human p16 gene into p16-null human glioma cell line, U251MG cells, using the plasmid vector pRc/CMV-p16 and evaluated the effect of p16 gene transfer on the growth suppression of malignant glioma cells. The transfection of p16 cDNA caused growth suppression through G1 cell cycle arrest in U251MG cells. We also examined the effect of p16 gene transfer on the chemosensitivity to cis-diamminedichloroplatinum II (CDDP), 1-(4-amino-2-methyl-5-pyrimidinyl)methyl-3-(2-chloroethyl) -3-nitrosourea hydrochloride (ACNU), and 5'-azacytidine (AZC). We did not detect any change in them after p16 gene transfer. These results might suggest that deletion of p16 genes promoted unrestrained growth in human glioma but has no relationship to the chemosensitivity to CDDP, ACNU and AZC.