Improved cell infiltration of highly porous nanofibrous scaffolds formed by combined fiber-fiber charge repulsions and ultra-sonication.
Improved cell infiltration of highly porous nanofibrous scaffolds formed by combined fiber-fiber charge repulsions and ultra-sonication.
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DOI：
10.1039/c4tb01487a
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发表时间：
2014-12-14
期刊：
影响因子：
--
通讯作者：
Alsberg E
中科院分区：
文献类型：
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作者：
Jeong SI;Burns NA;Bonino CA;Kwon IK;Khan SA;Alsberg E
影响电纺纳米纤维组织支架的一个重要问题是细胞对其三维(3D)结构的渗透性差。然而，环境和物理操作可以增强细胞对电纺支架的渗透。在本工作中，通过高湿度电纺和后处理超声处理相结合的方法，获得了厚度和孔隙率都有所增加的RGD改性海藻酸盐席。RGD改性的海藻酸盐、聚氧乙烯(PEO)和FDA批准的非离子表面活性剂混合物在20%和50%的相对湿度条件下进行电纺。在高湿度条件下电纺板坯，可显著增加板坯厚度，减小纤维直径。然后通过离子交联法和PEO/表面活性剂萃取法分离得到海藻酸盐含量。最后，对纯藻酸盐席进行超声后处理，进一步提高其横截面厚度。通过将成纤维细胞种植到藻酸盐垫子上来评估细胞的形态、增殖和渗透到支架中。随着湿度的增加和超声波的作用，细胞的铺展、生长和渗透能力都有所改善。这一方法为组织工程应用的细胞渗透性纳米纤维支架的设计带来了巨大的希望。
A significant problem affecting electrospun nanofibrous tissue scaffolds is poor infiltration of cells into their three-dimensional (3D) structure. Environmental and physical manipulation, however, can enhance cellular infiltration into electrospun scaffolds. In this work, RGD-modified alginate mats with increased thickness and porosity were achieved by pairing high humidity electrospinning with post-processing ultra-sonication. RGD-modified alginate, polyethylene oxide (PEO), and an FDA-approved, nonionic surfactant blends were electrospun in 20 and 50% relative humidity conditions. Mats electrospun in high humidity conditions resulted in significantly increased mat thickness and decreased fiber diameters. The mats’ alginate content was then isolated via ionic crosslinking and PEO/surfactant extraction. Finally, the alginate-only mat was post-processed by ultra-sonication to further enhance its cross-sectional thickness. Cell morphology, proliferation, and infiltration into the scaffolds were evaluated by seeding fibroblasts onto the alginate mat. Cell spreading, growth and infiltration improved with increased humidity and ultra-sonication. This approach shows great promise for the design of cell-permeable nanofibrous scaffolds for tissue-engineering applications.