The nuclear receptor homologue Ftz-F1 and the homeodomain protein Ftz are mutually dependent cofactors
The nuclear receptor homologue Ftz-F1 and the homeodomain protein Ftz are mutually dependent cofactors
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DOI：
10.1038/385548a0
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发表时间：
1997-02-06
期刊：
影响因子：
64.8
通讯作者：
Ephrussi, A
中科院分区：
文献类型：
--
作者：
Guichet, A;Copeland, JWR;Ephrussi, A
核激素受体和同源结构域蛋白是调节主要发育过程的两类转录因子。两者都依赖于与其他蛋白质的相互作用来获得特异性和活性。果蝇基因fushi tarazu（ftz）编码一种同源结构域蛋白（1）（Ftz），在发育胚胎中形成交替片段（2）需要合子。在这里，我们发现孤儿核受体α Ftz-F1（参考文献3），在卵子发生过程中沉积在卵子中（4），是Ftz的一个强制性辅因子。这两种蛋白质通过Ftz多肽中的保守结构域在体外和体内特异性地和直接地相互作用。这种相互作用表明，其他核受体/同源结构域蛋白质的相互作用可能是重要的和常见的发育中的生物体。
Nuclear hormone receptors and homeodomain proteins are two classes of transcription factor that regulate major developmental processes. Both depend on interactions with other proteins for specificity and activity. The Drosophila gene fushi tarazu (ftz), which encodes a homeodomain protein(1) (Ftz), is required zygotically for the formation of alternate segments in the developing embryo(2). Here we show that the orphan nuclear receptor alpha Ftz-F1 (ref. 3), which is deposited in the egg during oogenesis(4), is an obligatory cofactor for Ftz. The two proteins interact specifically and directly, both in vitro and in vivo, through a conserved domain in the Ftz polypeptide. This interaction suggests that other nuclear receptor/homeodomain protein interactions may be important and common in developing organisms.