HORMONE-RELEASE BY ISLET B-CELL-ENRICHED AND A-CELL-ENRICHED AND D-CELL-ENRICHED POPULATIONS PREPARED BY FLOW-CYTOMETRY
HORMONE-RELEASE BY ISLET B-CELL-ENRICHED AND A-CELL-ENRICHED AND D-CELL-ENRICHED POPULATIONS PREPARED BY FLOW-CYTOMETRY
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DOI：
10.1210/endo-113-5-1791
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发表时间：
1983-01-01
期刊：
影响因子：
4.8
通讯作者：
WEIR, GC
中科院分区：
文献类型：
--
作者：
FLETCHER, DJ;GROGAN, WM;WEIR, GC
用流式细胞术对分散的[大鼠]胰岛细胞进行低前角光散射分析。细胞产生明显的光散射模式，这似乎是细胞大小的函数，而不是细胞粒度的函数。放射免疫分析法（RIA）对从不同区域收集的细胞进行激素含量分析，发现含有胰高血糖素和生长抑素的细胞集中在散射强度较低的区域，而含有胰岛素的细胞则集中在散射强度较高的区域。与原始细胞悬液相比，这些制剂的胰高血糖素和生长抑素含量增加了3倍，胰岛素含量增加了6倍。在培养4 d前后分别检测完整胰岛细胞、未分选分散细胞和分选分散细胞的功能。在培养前，与完整的胰岛相比，所有分散的细胞群的基础分泌都有所增加，并且对葡萄糖、精氨酸或3-异丁基-1-甲基黄嘌呤的刺激浓度没有反应。培养4天后，基底分泌下降，反应性恢复。在A/D细胞富集和。细胞富集培养群体，单细胞百分比约为95%。这些人群的胰岛素释放模式与完整的胰岛和未分类的分散细胞相似。所有分散的细胞群释放的胰高血糖素远远超过完整的胰岛。显然，胰岛的结构组织影响A细胞的功能，但对β的影响明显。-细胞功能尚未得到证实。
Dispersed [rat] pancreatic islet cells were analyzed for their low forward angle light scatter using flow cytometry. The cells produced a distinct light scatter pattern which appeared to be a function of cell size and not cell granularity. RIA [radioimmunoassay] of hormone content of cells collected from different regions of the pattern revealed that glucagon- and somatostatin-containing cells were concentrated in regions of lower scatter intensity and that insulin-containing cells were more numerous in regions of higher intensity. Relative to the original cell suspension, these preparations were enriched 3-fold in glucagon and somatostatin content and 6-fold in insulin content. The function of intact islets, unsorted dispersed cells and sorted dispersed cells was examined before and after 4 days of culture. Before culture, all of the dispersed cell populations had elevated basal secretion compared with intact islets and did not respond to stimulatory concentrations of glucose, arginine or 3-isobutyl-1-methylxanthine. After culture for 4 days, basal secretion fell, and responsiveness returned. In both the A/D cell-enriched and the .beta. cell-enriched cultured populations, the percentage of single cells was approximately 95%. The insulin release patterns from these populations were similar to those from intact islets and unsorted dispersed cells. Glucagon release from all of the dispersed cell populations far exceeded that from intact islets. Apparently the structural organization of islets influences A cell function, but a clear influence upon .beta.-cell function has not been demonstrated.