Silkworm Midgut Proteins Interacting with a Hemolymph Protease Inhibitor, CI-8
Silkworm Midgut Proteins Interacting with a Hemolymph Protease Inhibitor, CI-8
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DOI：
10.1271/bbb.50548
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发表时间：
2006-07
期刊：
影响因子：
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通讯作者：
Y. Ueno;Ningjia He;M. Ujita;Kohji Yamamoto;Y. Banno;H. Fujii;Y. Aso
中科院分区：
文献类型：
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作者：
Y. Ueno;Ningjia He;M. Ujita;Kohji Yamamoto;Y. Banno;H. Fujii;Y. Aso
CI-8是家蚕血淋巴中的胰凝乳蛋白酶抑制剂。它发生在中肠在纺纱阶段的幼虫，但很少有信息的机制，其吸收中肠是可用的。我们发现与CI-8相互作用的两种多肽位于中肠膜中，并且我们使用生物素化的CI-8，即，p29和p60，分子大小分别为29 kDa和60 kDa。p29和p60的结构通过N-末端氨基酸测序和肽质量图谱（包括胰蛋白酶消化）进行检查。p29与血淋巴成熟的19 G1 - 30 K脂蛋白高度相似，而p60是新的。纯化的p29被抗19 G1 - 30 K抗体识别，并被证实与19 G1 - 30 K相似。该抗体还中和了中肠膜中p29的Cl-8结合能力。p29和p60可能是参与CI-8通过膜摄取到中肠中的蛋白质因子。
CI-8 is the chymotrypsin inhibitor in hemolymph from the silkworm, Bombyx mori. It occurs in the midgut at the spinning stage of larva, but little information on the mechanism of its uptake in the midgut is available. We found that two polypeptides interacting with CI-8 are in the midgut membrane, and we purified them using a biotinylated CI-8, viz., p29 and p60, having molecular sizes of 29 kDa and 60 kDa respectively. The structures of p29 and p60 were examined by N-terminal amino acid sequencing and peptide mass mapping, including tryptic digestion. p29 was highly similar to the matured 19G1-30K lipoprotein from hemolymph, but p60 was novel. Purified p29 was recognized by anti-19G1-30K antibody, and was confirmed to be similar to 19G1-30K. The antibody also neutralized the CI-8 binding ability of p29 in the midgut membrane. p29 and p60 are perhaps proteinaceous factors involved in the uptake of CI-8 into the midgut through the membrane.