Control of the heat stress-induced alternative splicing of a subset of genes by hnRNP K.
Control of the heat stress-induced alternative splicing of a subset of genes by hnRNP K.
复制标题
hnRNP K 对热应激诱导的基因子集选择性剪接的控制。
DOI：
10.1111/gtc.12400
复制
发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Inoue K.
中科院分区：
文献类型：
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作者：
Yamamoto K;Furukawa M;Fukumura K;Kawamura A;Yamada T;Suzuki H;Hirose T;Sakamoto H;Inoue K.
前mRNA剪接在真核细胞中热休克时被广泛抑制。然而，研究表明热应激时HSP 105前mRNA会发生选择性剪接。利用HeLa细胞中的RNAi筛选，我们发现RNA结合蛋白hnRNP K和PSF/SFPQ是热应激期间HSP 105外显子12排除所必需的。此外，外显子阵列分析表明，一组基因在热应激期间以hnRNP K依赖性方式选择性剪接，而hnRNP K对于其余基因的应激诱导的选择性剪接不是必需的。在后一组中，我们发现SRp 38/SRSF 10和SC 35/SRSF 2是热应激时TNRC 6A外显子13包含所必需的。因此，我们的研究清楚地表明，几种RNA结合蛋白参与了哺乳动物细胞中响应热应激的剪接调节。
Pre‐mRNA splicing is widely repressed upon heat shock in eukaryotic cells. However, it has been shown thatHSP105pre‐mRNA is alternatively spliced in response to heat stress. Using RNAi screening in HeLa cells, we found that RNA‐binding proteins hnRNP K and PSF/SFPQ are necessary for the exon 12 exclusion ofHSP105during heat stress. Moreover, exon array analyses showed that a group of genes is alternatively spliced during heat stress in an hnRNP K‐dependent manner, whereas hnRNP K is not necessary for the stress‐induced alternative splicing of the remaining genes. Among the latter group, we found that SRp38/SRSF10 and SC35/SRSF2 are necessary for the inclusion of exon 13 ofTNRC6Aduring heat stress. Thus, our study clearly showed that several RNA‐binding proteins are involved in the splicing regulation in response to heat stress in mammalian cells.