Effect of Oxidizing Agents and Sulfhydryl Group Reagents on Beta Toxin from Clostridium perfringens Type C
Effect of Oxidizing Agents and Sulfhydryl Group Reagents on Beta Toxin from Clostridium perfringens Type C
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氧化剂和巯基试剂对C型产气荚膜梭菌β毒素的影响
DOI：
10.1111/j.1348-0421.1980.tb02862.x
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发表时间：
1980
影响因子：
2.6
通讯作者：
M. Matsuura
中科院分区：
文献类型：
--
作者：
J. Sakùrai;Y. Fujii;M. Matsuura
C 型产气荚膜梭菌的纯化 β 毒素可被氧化剂邻碘代苯甲酸酯 (OIBA)、氧化型谷胱甘肽和铁氰化物以及巯基试剂 5,5'-二硫代双(2-硝基苯甲酸) (DTNB) 和 N-乙基马来酰亚胺、碘乙酰胺和碘乙酸灭活，导致不同程度的活性丧失，具体取决于使用浓度。毒素的活性不受暴露于 1.0 mM 对氯汞苯甲酸酯的影响。经过 OIBA 或 DTNB 处理的毒素通过与 2-巯基乙醇和二硫苏糖醇一起孵育而被重新激活。数据表明，β 毒素含有对其活性至关重要的硫醇基团。
Purified beta toxin from Clostridium perfringens type C was inactivated by the oxidizing agents o‐iodosobenzoate (OIBA), oxidized glutathione, and ferricyanide, and by the sulfhydryl group reagents 5,5′‐dithio‐bis(2‐nitrobenzoic acid) (DTNB) and N‐ethylmaleimide, iodoacetamide, and iodoacetic acid, causing loss of activity in various degrees depending on the concentration used. The activity of the toxin was not influenced by exposure to 1.0 mM of p‐chloromercuribenzoate. The toxin treated by OIBA or DTNB was reactivated by incubation with 2‐mercaptoethanol and dithiothreitol. The data suggest that beta toxin contains thiol groups which are essential for the activity.