Partial characterization of the soluble mediator of leukocyte adherence inhibition.
Partial characterization of the soluble mediator of leukocyte adherence inhibition.
复制标题
白细胞粘附抑制的可溶性介质的部分表征。
作者：
T. Appelboom;M. Piérart;G. Ameryckx;N. Mairesse
关节炎白细胞粘附抑制（LAI）试验是单核细胞、T细胞和多形核白细胞（PMN）之间协同作用的模型。来自类风湿性关节炎患者的单核细胞（MNC）在与聚集的IgG接触的60分钟内在试管中释放能够抑制PMN对玻璃的粘附（LAI活性）的上清液。通过蛋白质标记、分级分离和电印迹，我们发现上清的LAI活性位于凝胶的14-20 kDa区域，并且在MNC刺激时被主动分泌。在负离子条件下，具有LAI活性的因子能够形成较大的复合物，并且它们的电荷是不均匀的。它们与白细胞介素-1（IL-1）的关系进行了讨论，因为上清液在胸腺细胞增殖试验中给出了阳性反应;少量的IL-1具有抑制PMN增殖的能力，抗IL-1阻断了这种活性。
The leukocyte adherence inhibition (LAI) test in arthritis is a model of cooperation between monocytes, T cells and polymorphonuclear cells (PMN). Mononuclear cells (MNC) from patients with rheumatoid arthritis in contact with aggregated IgG release within 60 min in the test tube a supernatant capable of inhibiting the adherence of PMN to glass (LAI activity). By protein labeling, fractionation and electroblotting, we showed that the supernatant LAI activity was located in the 14-20 kDa region of the gel and was actively secreted upon MNC stimulation. Under negative conditions, the factors with LAI activity were able to constitute larger complexes and their charge was heterogeneous. Their relationship to interleukin-1 (IL-1) is discussed, since the supernatant gave a positive response in the thymocyte proliferation assay; small amounts of IL-1 had the capacity to inhibit PMN adhesiveness and anti-IL-1 blocked this activity.