Expression of full-length trkB receptors by reactive astrocytes after chronic CNS injury.
Expression of full-length trkB receptors by reactive astrocytes after chronic CNS injury.
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慢性中枢神经系统损伤后反应性星形胶质细胞表达全长 trkB 受体。
DOI：
10.1006/exnr.1997.6698
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Large,TH
中科院分区：
文献类型：
--
作者：
McKeon,RJ;Silver,J;Large,TH
生长因子，包括神经营养蛋白家族的成员，在中枢神经系统损伤后由神经元和神经胶质元件表达。为了评估神经胶质细胞在慢性损伤部位对神经营养因子作出反应的能力，将硝化纤维滤器植入大脑皮层 30 天后，对全长 trkB 受体进行定位。 Northern 分析表明，过滤器植入物含有表达全长和截短 trkB 受体转录本的细胞，与培养的星形胶质细胞主要表达截短的 trkB 受体相反。使用 trkB 激酶结构域探针进行原位杂交和免疫组织化学，将全长受体与过滤器植入物附近和内部的 GFAP 免疫阳性反应性星形胶质细胞共定位。相反，OX-42 免疫阳性小胶质细胞/巨噬细胞未对全长 trkB 进行染色。这些数据表明，在大脑皮层受到慢性损伤后，反应性星形胶质细胞可以表达功能性 trkB 受体，并支持神经营养素可能调节星形胶质细胞对损伤反应的假设。
Growth factors, including members of the neurotrophin family, are expressed by neuronal and glial elements following injury to the CNS. In order to assess the capacity for glial cells to respond to neurotrophins at sites of chronic injury, full-length trkB receptors were localized following implantation of a nitrocellulose filter into the cerebral cortex for 30 days. Northern analysis demonstrated that filter implants contained cells expressing transcripts for full-length and truncated trkB receptors, in contrast to the predominant expression of truncated trkB receptors by cultured astrocytes.In situhybridization and immunohistochemistry using probes to the trkB kinase domain colocalized full-length receptors with GFAP-immunopositive reactive astrocytes adjacent to and within the filter implant. In contrast, OX-42-immunopositive microglia/macrophages were not stained for full-length trkB. These data indicate that reactive astrocytes can express functional trkB receptors following a chronic insult to the cerebral cortex and support the hypothesis that neurotrophins may regulate astrocytic responses to injury.