Effect of dietary state on hepatocyte size
Effect of dietary state on hepatocyte size
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DOI：
10.1007/bf01122168
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发表时间：
1982-12
影响因子：
4
通讯作者：
B. Uhal;K. Roehrig
中科院分区：
文献类型：
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作者：
B. Uhal;K. Roehrig
为了计算细胞代谢物浓度，有必要准确测定细胞体积。本文报告的实验旨在确定饮食状态对从进食、禁食（48 h）或禁食（48 h）-再喂食（48 h）雄性Sprague-Dawley大鼠肝脏中分离的肝细胞体积的影响。通过使用光学测微计容易且快速地获得的细胞直径来计算基本上球形肝细胞的细胞体积。禁食导致肝细胞体积减少约2倍，再喂食导致恢复至对照值的83%。蛋白质含量/1000个细胞在禁食时也下降了两倍，但在再喂食时恢复到对照值的70%。这种快速，简单的方法来确定肝细胞体积同意以及通过更费力的手段获得的数据。
In order to calculate cellular metabolite concentrations, it is necessary to determine accurately the cellular volume. The purpose of the experiments reported here was to determine the effect of dietary state on the volume of hepatocytes isolated from livers of fed, fasted (48 h) or fasted (48 h)-refed (48 h) male Sprague-Dawley rats. Cellular volumes of the essentially spherical hepatocytes were calculated from the diameters of the cells obtained easily and rapidly by using an optical micrometer. Fasting resulted in approximately a two-fold reduction in hepatocyte volume, and refeeding resulted in a return to 83% of the control value. The protein content/1000 cells also dropped two-fold upon fasting but returned to 70% of the control value upon refeeding. This rapid, simple method for determining hepatocyte volumes agrees well with data obtained by more laborious means.