Experimental transmission of human subacute spongiform encephalopathy to small rodents
Experimental transmission of human subacute spongiform encephalopathy to small rodents
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人类亚急性海绵状脑病向小型啮齿动物的实验传播
DOI：
10.1007/bf00690454
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发表时间：
2004
影响因子：
12.7
通讯作者：
M. Ohta
中科院分区：
文献类型：
--
作者：
J. Tateishi;Y. Sato;M. Koga;H. Doi;M. Ohta
本文报告了三例人的亚急性海绵状脑病对小型啮齿类动物的实验性传播。第一例非典型克雅氏病伴海绵状改变、库鲁斑和白质软化，直接传播给小鼠、大鼠和豚鼠，并通过大鼠间接传播给仓鼠和蒙古沙鼠。从另外两个典型的SSE病例中，疾病也成功传播;从第二个病例到小鼠和大鼠，从第三个病例到豚鼠。脑显示出最高的感染性;病动物的脾、肝、血液和脑脊液也具有感染性。脑内接种是传播最快的途径，其次是鞘内、腹膜内、粘膜下和皮下途径。孵育期和临床特征在每种接种种属中具有特征性，并且在几次传代中没有变化，但从第一次到第二次传代的孵育期缩短。组织学上，在所有患病动物中观察到显著的海绵状状态和星形胶质细胞增殖，尽管病变的分布是每个物种特有的。小鼠白色物质的严重病变与接种羊瘙痒病的小鼠相似，也与第一个病例相似。
Experimental transmission of subacute spongiform encephalopathy from three human cases to small rodents is reported. The first case with atypical CJD with spongiform change, kuru plaques, and leukomalacia was transmitted directly to mice, rats, and guinea pigs and indirectly to hamsters and Mongolian gerbils through rats. From two other typical SSE cases the disease was also successfully transmitted; from the second case to mice and rats, and from the third case to guinea pigs. Brain showed the highest infectivity; the spleen, liver, blood, and cerebrospinal fluid of diseased animals were also infective. Intracerebral inoculation was the route for the fastest transmission, followed by intrathecal, intraperitoneal, submucosal, and subcutaneous routes. The incubation periods and clinical features were characteristic in each inoculated species and did not vary within several passages, except for the shortening of incubation period from the first to the second passage. Histologically, a marked spongy state and proliferation of astrocytes were observed in all diseased animals, though the distribution of the lesion was peculiar to each species. The severe lesion in the white matter in mice was similar to that seen in mice inoculated with scrapie and also to that seen in the first case.