Induced cell proliferation in putative haematopoietic tissues of the sea star, Asterias rubens (L.)
Induced cell proliferation in putative haematopoietic tissues of the sea star, Asterias rubens (L.)
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DOI：
10.1242/jeb.018507
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发表时间：
2008-08-15
影响因子：
2.8
通讯作者：
Hernroth, Bodil
中科院分区：
文献类型：
--
作者：
Holm, Kristina;Dupont, Samuel;Hernroth, Bodil
棘皮动物Asterias Rubens的体腔液中含有大量的循环体腔细胞。本研究旨在扩大对这些细胞的造血源的认识。注射免疫刺激分子脂多糖(LPS)和刀豆蛋白A(ConA)可导致体腔细胞数量增加。为了研究这些分子是否会在假定的造血组织(HPTs)中诱导细胞增殖，我们进行了替代核苷酸5-溴-2‘-脱氧尿苷(BrdU)的短期暴露。用荧光素标记的抗体追踪BrdU的免疫组织化学分析显示，体腔上皮和轴器官中有明显的细胞分裂。在幽门盲囊中，未检测到增殖。胶原酶处理的体腔上皮、中轴器和铁氏小体的单层细胞BrdU标记显示，LPS和ConA均可诱导细胞增殖，而幽门盲囊和循环体腔细胞的增殖仍很稀疏。用共聚焦显微镜观察到，体腔上皮外植体释放的细胞的形态和功能行为与循环吞噬细胞有很高的相似性。结论：内毒素和刀豆素A诱导的体腔上皮、轴器和蒂德曼小体的细胞增殖反映了体腔细胞数量的增加，这进一步证实了这些器官是HPTs的观点和体细胞更新的来源。
The coelomic fluid of the echinoderm Asterias rubens possesses large populations of circulating coelomocytes. This study aimed to expand the knowledge about the haematopoietic sources of these cells. Injection of the immune-stimulating molecules lipopolysaccharide (LPS) and concanavalin A (ConA) resulted in an increase in coelomocytes. To investigate if these molecules induce cell proliferation in putative haematopoietic tissues (HPTs), short-term exposure of the substitute nucleotide 5-bromo-2'-deoxyuridine (BrdU) was conducted. Immunohistochemical analysis, using fluorescein-labelled antibodies to trace BrdU, showed pronounced cell division in the coelomic epithelium and axial organ. In the pyloric caeca, not considered as an HPT, proliferation was not detected. BrdU labelling of monolayers of cells obtained by collagenase treatment of coelomic epithelium, axial organ and Tiedemann body revealed induced cell proliferation in response to both LPS and ConA while proliferation of pyloric caeca and circulating coelomocytes remained sparse. By using confocal microscopy it was observed that both the morphology and functional behaviour of cells released from explants of coelomic epithelium showed high similarity to those of circulating phagocytes. It was concluded that the increased coelomocyte numbers observed in response to LPS and ConA were reflected in an induced cell proliferation in coelomic epithelium, axial organ and Tiedemann body, which reinforces the idea that these organs are HPTs and the sources of coelomocyte renewal.